Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans.

Journal of Clinical Microbiology
Wenlan ZhangHelge Karch

Abstract

By using sequence analysis of Shiga toxin 1 (Stx 1) genes from human and ovine Stx-producing Escherichia coli (STEC) strains, we identified an Stx1 variant in STEC of human origin that was identical to the Stx1 variant from ovine STEC, but demonstrated only 97.1 and 96.6% amino acid sequence identity in its A and B subunits, respectively, to the Stx1 encoded by bacteriophage 933J. We designated this variant "Stx1c" and developed stxB(1) restriction fragment length polymorphism and stx(1c)-specific PCR strategies to determine the frequency and distribution of stx(1c) among 212 STEC strains isolated from humans. stx(1c) was identified in 36 (17.0%) of 212 STEC strains, 19 of which originated from asymptomatic subjects and 16 of which were from patients with uncomplicated diarrhea. stx(1c) was most frequently (in 23 STEC strains [63.9%]) associated with stx(2d), but 12 (33.3%) of the 36 STEC strains possessed stx(1c) only. A single STEC strain possessed stx(1c) together with stx(2) and was isolated from a patient with hemolytic-uremic syndrome. All 36 stx(1c)-positive STEC strains were eae negative and belonged to 10 different serogroups, none of which was O157, O26, O103, O111, or O145. Stx1c was produced by all stx(1c)-containin...Continue Reading

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Citations

Jun 7, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Kiyotaka Nishikawa
Aug 16, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Johanna ScheiringLothar Bernd Zimmerhackl
Aug 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alexander MellmannHelge Karch
Apr 18, 2012·Foodborne Pathogens and Disease·Beatriz A D'AstekMarta Rivas
Dec 15, 2012·Foodborne Pathogens and Disease·Aine M MonaghanDeclan J Bolton
Jul 24, 2007·Applied and Environmental Microbiology·Murilo G OliveiraKinue Irino
Jan 1, 2013·Applied and Environmental Microbiology·Dongjin ParkCharles W Kaspar
Oct 18, 2011·Applied and Environmental Microbiology·Aine MonaghanDeclan J Bolton
Mar 5, 2005·Applied and Environmental Microbiology·Abram AertsenChris W Michiels
Jan 17, 2004·Clinical Microbiology Reviews·Herbert Schmidt, Michael Hensel
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien
Feb 15, 2008·Journal of Clinical Microbiology·Wenlan ZhangThorsten Kuczius
Nov 28, 2002·Journal of Clinical Microbiology·Marjut EklundAnja Siitonen
Nov 8, 2003·Journal of Clinical Microbiology·Vidiya RamachandranSteven P Djordjevic
May 8, 2003·Journal of Clinical Microbiology·Christine BürkErwin Märtlbauer
Mar 9, 2004·Journal of Clinical Microbiology·Anne-Katharina SonntagHelge Karch
Mar 10, 2010·Future Microbiology·Vernon L Tesh
Apr 4, 2012·Emerging Infectious Diseases·Taru LienemannAnja Siitonen
Nov 10, 2011·Toxins·Elisabete ValérioRogério Tenreiro
Oct 10, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Martina BielaszewskaHelge Karch

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