Identification, characterization, and expression of a novel alpha-tropomyosin isoform in cardiac tissues in developing chicken

Journal of Cellular Biochemistry
Robert W ZajdelDipak K Dube

Abstract

Tropomyosins are present in various muscle (skeletal, cardiac, and smooth) and non-muscle cells with different isoforms characteristic of specific cell types. We describe here a novel smooth/striated chimeric isoform that was expressed in developing chick heart in addition to the classically described TM-4 type. This novel alpha-Tm tropomyosin isoform, designated as alpha-Tm-2, contains exon 2a (in place of exon 2b). The known striated muscle isoform (alpha-Tm-1) was also expressed in embryonic hearts along with the striated muscle isoform of TM-4. In adult heart, TM-4 was expressed, however, expression of both alpha-Tm-1 and alpha-Tm-2 isoforms was drastically reduced or downregulated. Interestingly, we were unable to detect the expression of alpha-Tm-2 in embryonic and adult skeletal muscle, however, the alpha-Tm-1 isoform is expressed in embryonic and adult skeletal muscle. Examination of other possible isoforms of the alpha-TM gene, i.e., alpha-smooth muscle tropomyosin (alpha-Sm), alpha-Fibroblast-1 (alpha-F1), and alpha-Fibroblast-2 (alpha-F2) revealed expression in embryonic hearts and a significant reduction of each of these isoforms in adult heart. In order to elucidate the role of the newly discovered tropomyosin isof...Continue Reading

References

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Citations

May 31, 2014·Molecular Biology International·Syamalima DubeDipak K Dube
Apr 1, 2017·Journal of Molecular and Cellular Cardiology·Jennifer EnglandSiobhan Loughna
Dec 9, 2017·Cytoskeleton·Syamalima DubeDipak K Dube
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Dipak K DubeJoseph W Sanger
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Dipak K DubeBernard J Poiesz
Aug 21, 2007·Cell Motility and the Cytoskeleton·Jushuo WangJoseph W Sanger
Feb 28, 2008·Cell Motility and the Cytoskeleton·Jushuo WangJoseph W Sanger
Jun 22, 2010·Journal of Cellular Biochemistry·Anish ThomasDipak K Dube
Dec 1, 2013·Regenerative Medicine Research·Robert W ZajdelDipak K Dube
Oct 25, 2011·Cytoskeleton·Jushuo WangJoseph W Sanger

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