PMID: 8950284Jan 1, 1996Paper

Identification, characterization and localization of corticotropin-releasing hormone receptors in human placenta

Life Sciences
A HatzoglouE Castanas

Abstract

The purpose of this work was to identify, characterise, and localise specific CRH binding sites in whole vaginally delivered term human placental membranes as well as in dispersed and purified trophoblastic membranes. We have found that whole placenta membranes contained specific and saturable CRH-binding sites. Maximum specific binding was obtained at pH 7.2-7.4, 22 C, for 2 h. The order of potency of CRH analogs was h/rCRH > alpha-helCRH > oCRH. Scatchard analysis revealed a single population of high affinity binding sites with a dissociation constant of 1.25 nM and maximum binding capacity of 119 fmol/mg of protein. Purified syncytiotrophoblast membranes contained high affinity CRH binding sites exhibiting the same dissociation constant and maximum binding capacity as whole placental membranes, suggesting that the CRH binding sites are expressed almost exclusively by the syncytiotrophoblast. Our findings indicate that CRH binding sites in the human placenta are exhibit similar characteristics to that described for anterior pituitary, brain and adrenals.

References

Feb 28, 1989·Biochemical and Biophysical Research Communications·S A Jones, J R Challis
May 1, 1988·The Journal of Clinical Endocrinology and Metabolism·A N MargiorisG P Chrousos
Nov 13, 1987·Biochemical and Biophysical Research Communications·M GrinoA N Margioris
Feb 1, 1987·The Journal of Clinical Endocrinology and Metabolism·A SasakiK Yoshinaga
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R ChenW W Vale
Mar 1, 1994·Molecular and Cellular Endocrinology·D GrammatopoulosE W Hillhouse

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Citations

Feb 13, 2001·European Journal of Clinical Investigation·B SaeedC Self
Sep 14, 2012·Reproductive Biology and Endocrinology : RB&E·Fabian B FahlbuschJörg Dötsch
May 1, 2010·The Journal of Veterinary Medical Science·Ryosuke SakumotoTokushi Komatsu
Apr 13, 1999·The Journal of Clinical Endocrinology and Metabolism·F PetragliaW Vale
Jul 16, 2010·Endocrine Reviews·Felice PetragliaJohn R G Challis
Jun 8, 2000·The Journal of Clinical Endocrinology and Metabolism·E KarterisE W Hillhouse
Jun 24, 2011·Journal of Reproductive Immunology·Gabriela WypiorJulia Szekeres-Bartho

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