PMID: 9430619Feb 28, 1998Paper

Identification, characterization and partial purification of a thiol-protease which cleaves specifically the skeletal muscle ryanodine receptor/Ca2+ release channel

The Journal of Membrane Biology
S ShevchenkoV Shoshan-Barmatz

Abstract

A 94 kDa large subunit thiol-protease, as identified by anti-calpain antibodies, has been isolated from skeletal muscle junctional sarcoplasmic reticulum (SR). This protease cleaves specifically the skeletal muscle ryanodine receptor (RyR)/Ca2+ release channel at one site resulting in the 375 kDa and 150 kDa fragments. The 94 kDa thiol-protease degrades neither other SR proteins nor the ryanodine receptor of cardiac nor brain membranes. The partially purified 94 kDa protease, like the SR associated protease, had an optimal pH of about 7.0, was absolutely dependent on the presence of thiol reducing reagents, and was completely inhibited by HgCl2, leupeptin and the specific calpain I inhibitor. However, while the SR membrane-associated protease requires Ca2+ at a submicromolar concentration, the isolated thiol-protease has lost the Ca2+ requirement. The 94 kDa thiol-protease had no effect on ryanodine binding but modified the channel activity of RyR reconstituted into planar lipid bilayer: in a time-dependent manner, the channel activity decreases and within several minutes the channel is converted into a subconducting state. The protease-modified channel activity is still Ca2+-dependent and ryanodine sensitive. This 94 kDa thiol...Continue Reading

Citations

Sep 24, 1999·Journal of Neuroscience Research·S L Chan, M P Mattson
Mar 29, 2003·Journal of Chemical Neuroanatomy·Norbert KönigYves Benyamin
Sep 11, 2002·Neuromuscular Disorders : NMD·Urs T RueggTimo M Buetler
Sep 13, 2001·Journal of Neurochemistry·R P GuttmannD R Lynch
Aug 6, 2011·Circulation Research·Cam PattersonMonte S Willis
Feb 22, 2014·Journal of Muscle Research and Cell Motility·Keita KanzakiMasanobu Wada
Jan 1, 2009·International Journal of Cell Biology·Govindan DayanithiStephen Baghdiguian
Nov 18, 2015·Proceedings of the National Academy of Sciences of the United States of America·Nicolas PlaceHåkan Westerblad
Dec 5, 2006·Environmental Pollution·S WojasD M Antosiewicz
Aug 17, 2002·The Journal of Pharmacology and Experimental Therapeutics·Rodney P GuttmannDavid R Lynch
Oct 28, 2014·Physiological Reports·Cory W BaumannChristopher P Ingalls
Aug 9, 2002·The Journal of Biological Chemistry·Kalanethee Paul-PletzerJerome Parness
Jan 11, 2019·Physiological Research·S FilipG Dayanithi
Aug 16, 2000·American Journal of Physiology. Cell Physiology·K R SultanD Pette

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