Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering

Microbial Cell Factories
Huiling ChengHairong Cheng

Abstract

Erythritol is a four-carbon sugar alcohol with sweetening properties that is used by the agro-food industry as a food additive. In the yeast Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose reductase(s). In the earlier report, an erythrose reductase gene (YALI0F18590g) from erythritol-producing yeast Y. lipolytica MK1 was identified (Janek et al. in Microb Cell Fact 16:118, 2017). However, deletion of the gene in Y. lipolytica MK1 only resulted in some lower erythritol production but the erythritol synthesis process was still maintained, indicating that other erythrose reductase gene(s) might exist in the genome of Y. lipolytica. In this study, we have isolated genes g141.t1 (YALI0D07634g) and g3023.t1 (YALI0C13508g) encoding two novel erythrose reductases (ER). The biochemical characterization of the purified enzymes showed that they have a strong affinity for erythrose. Deletion of the two ER genes plus g801.t1 (YALI0F18590g) did not prevent erythritol synthesis, suggesting that other ER or ER-like enzymes remain to be discovered in this yeast. Overexpression of the newly isolated two genes (ER10 or ER25) led to an average 14.7% higher erythritol yield and 31...Continue Reading

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Citations

Mar 18, 2020·Critical Reviews in Food Science and Nutrition·Muhammad BilalHairong Cheng
Apr 25, 2020·Microorganisms·Patrick FickersCarol Sze Ki Lin
Nov 22, 2019·Cell Biology International·Papa Makhona NiangPatrick Fickers
May 19, 2019·Applied Microbiology and Biotechnology·Yirong XuHairong Cheng
Feb 18, 2021·FEMS Yeast Research·Dmitry Mamaev, Renata Zvyagilskaya
Mar 30, 2021·Frontiers in Bioengineering and Biotechnology·Abdul Rahman AbbasiYongkun Lv

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Methods Mentioned

BETA
PCR
gel filtration
gel-filtration

Software Mentioned

BLAST
IPKSNNVER
Phoretics
MEGA7
OriginPro

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