Identification, Classification, and Expression Analysis of GRAS Gene Family in Malus domestica

Frontiers in Physiology
Sheng FanMingyu Han

Abstract

GRAS genes encode plant-specific transcription factors that play important roles in plant growth and development. However, little is known about the GRAS gene family in apple. In this study, 127 GRAS genes were identified in the apple (Malus domestica Borkh.) genome and named MdGRAS1 to MdGRAS127 according to their chromosomal locations. The chemical characteristics, gene structures and evolutionary relationships of the MdGRAS genes were investigated. The 127 MdGRAS genes could be grouped into eight subfamilies based on their structural features and phylogenetic relationships. Further analysis of gene structures, segmental and tandem duplication, gene phylogeny and tissue-specific expression with ArrayExpress database indicated their diversification in quantity, structure and function. We further examined the expression pattern of MdGRAS genes during apple flower induction with transcriptome sequencing. Eight higher MdGRAS (MdGRAS6, 26, 28, 44, 53, 64, 107, and 122) genes were surfaced. Further quantitative reverse transcription PCR indicated that the candidate eight genes showed distinct expression patterns among different tissues (leaves, stems, flowers, buds, and fruits). The transcription levels of eight genes were also inv...Continue Reading

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Citations

Jul 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abdullah ShalmaniMingyu Han
Aug 11, 2018·Genome Génome / Conseil National De Recherches Canada·Bowei YanJingyu Xu
Jan 31, 2020·BMC Plant Biology·Zhongying ShanYanmei Zhu
Dec 14, 2021·Frontiers in Plant Science·Mouboni DuttaPulugurtha Bharadwaja Kirti

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Datasets Mentioned

BETA
E-GEOD-42873

Methods Mentioned

BETA
AtLAS
PCR
transgenic

Software Mentioned

ExPaSy
MEGA
Primer
SPSS
Origin
MapDraw
PlantCARE
Circos
BLASTP
Gene Structure Display Server ( GSDS

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