Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napus L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death

DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes
Bisi ChenYuan-Qing Jiang

Abstract

The R2R3-MYB proteins comprise one of the largest families of transcription factors in plants. Although genome-wide analysis of this family has been carried out in some plant species, little is known about R2R3-MYB genes in canola (Brassica napus L.). In this study, we have identified 76 R2R3-MYB genes in the canola genome through mining of expressed sequence tags (ESTs). The cDNA sequences of 44 MYB genes were successfully cloned. The transcriptional activities of BnaMYB proteins encoded by these genes were assayed in yeast. The subcellular localizations of representative R2R3-MYB proteins were investigated through GFP fusion. Besides, the transcript abundance level analysis during abiotic conditions and ABA treatment identified a group of R2R3-MYB genes that responded to one or more treatments. Furthermore, we identified a previously functionally unknown MYB gene-BnaMYB78, which modulates reactive oxygen species (ROS)-dependent cell death in Nicotiana benthamiana, through regulating the transcription of a few ROS- and defence-related genes. Taken together, this study has provided a solid foundation for understanding the roles and regulatory mechanism of canola R2R3-MYB genes.

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Citations

Jul 14, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Darren Chern Jan WongJosé Tomás Matus
Jul 22, 2017·Genome Génome / Conseil National De Recherches Canada·Ali HajiebrahimiShiva Hemmati
Jul 28, 2019·The Plant Journal : for Cell and Molecular Biology·Yimin LiYiji Xia
Jul 16, 2019·Molecular Plant Pathology·Peter Balint-Kurti
Jul 8, 2020·The Plant Journal : for Cell and Molecular Biology·Fangfang NiuYuan-Qing Jiang
Jan 20, 2021·GM Crops & Food·Ali RazaMirza Hasanuzzaman
Sep 10, 2020·Plant Science : an International Journal of Experimental Plant Biology·Fernanda ValandroAlexandro Cagliari
May 14, 2021·Critical Reviews in Biotechnology·Yingjia TongJingwen Zhou

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