Identification, cloning and functional characterization of novel sperm associated antigen 11 (SPAG11) isoforms in the rat.

Reproductive Biology and Endocrinology : RB&E
Suresh YenuguSusan H Hall

Abstract

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family were found to play an important role in epididymal innate immunity in addition to their role in sperm maturation. However, the expression of Spag11 transcripts in rodents is not well documented. Computational analysis was employed to identify novel Spag11 isoforms in the rat. RT-PCR analyses were carried out on RNAs isolated from the male reproductive tract tissues of rat using gene specific primers for Spag11c and Spag11t. The identities of PCR products were confirmed by sequencing. Tissue distribution, developmental expression and androgen regulation of Spag11t and Spag11c were studied using RT-PCR. The antimicrobial activities of recombinant Spag11t and Spag11c were tested against E coli in a colony forming unit assay. In this study, we identified two novel Spag11 transcripts, namely, Spag11t and Spag11c derived from the long arm of chromosome 16 in the rat (Rattus norvegicus), using both in silico and molecular biology approaches. Spag11c is expressed in all three regions of the epididymis, in testis and in ovary but is absent from the seminal vesicle. Spag11t expression is confined to the caput and it is not expressed in...Continue Reading

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Citations

Jan 13, 2009·Human Reproduction Update·Gail A Cornwall
May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
Jun 19, 2009·Biology of Reproduction·Yashwanth RadhakrishnanFrank S French
Jun 26, 2007·Asian Journal of Andrology·Susan H HallFrank S French
Aug 10, 2007·Cell Motility and the Cytoskeleton·Jonathan D SteelsWilliam S Trimble
Sep 10, 2016·PloS One·Ganapathy Narmadha, Suresh Yenugu

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Datasets Mentioned

BETA
AY600144
AY600145

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPOT
PROSITE
PhotoShop
ExPASy
BLAST
MapView

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