Identification, evolution and expression of an insulin-like peptide in the cephalochordate Branchiostoma lanceolatum

PloS One
Claire LecroiseyVincent Laudet

Abstract

Insulin is one of the most studied proteins since it is central to the regulation of carbohydrate and fat metabolism in vertebrates and its expression and release are disturbed in diabetes, the most frequent human metabolic disease worldwide. However, the evolution of the function of the insulin protein family is still unclear. In this study, we present a phylogenetic and developmental analysis of the Insulin Like Peptide (ILP) in the cephalochordate amphioxus. We identified an ILP in the European amphioxus Branchiostoma lanceolatum that displays structural characteristics of both vertebrate insulin and Insulin-like Growth Factors (IGFs). Our phylogenetic analysis revealed that amphioxus ILP represents the sister group of both vertebrate insulin and IGF proteins. We also characterized both temporal and spatial expression of ILP in amphioxus. We show that ilp is highly expressed in endoderm and paraxial mesoderm during development, and mainly expressed in the gut of both the developing embryo and adult. We hypothesize that ILP has critical implications in both developmental processes and metabolism and could display IGF- and insulin-like functions in amphioxus supporting the idea of a common ancestral protein.

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Citations

May 3, 2019·Cell and Tissue Research·Satoshi NakayamaMichio Ogasawara
Aug 26, 2019·Cell and Tissue Research·Rossella AnnunziataMaria I Arnone
Nov 20, 2018·Frontiers in Endocrinology·Margherita PerilloMaria I Arnone
Dec 1, 2017·Nature Ecology & Evolution·Patrick R H SteinmetzUlrich Technau
Jan 11, 2019·Data in Brief·Maëva Cherif-FeildelKristell Kellner

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Methods Mentioned

BETA
PCR

Software Mentioned

MUSCLE
Guidance
PhyML
ClustalW
ProtTest
BestKeeper
MAFFT
CFX Manager

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