Identification, expression and characterisation of a major salivary allergen (Cul s 1) of the biting midge Culicoides sonorensis relevant for summer eczema in horses.

International Journal for Parasitology
Kathrin F A LangnerBarbara S Drolet

Abstract

Salivary proteins of Culicoides biting midges are thought to play a key role in summer eczema (SE), a seasonal recurrent allergic dermatitis in horses. The present study describes the identification, expression and clinical relevance of a candidate allergen of the North American midge Culicoides sonorensis. Immunoblot analysis of midge saliva revealed a 66 kDa protein (Cul s 1) that was bound by IgE from several SE-affected (SE+) horses. Further characterisation by fragmentation, mass spectrometry and bioinformatics identified Cul s 1 as maltase, an enzyme involved in sugar meal digestion. A cDNA encoding Cul s 1 was isolated and expressed as a polyhistidine-tagged fusion protein in a baculovirus/insect cell expression system. The clinical relevance of the affinity-purified recombinant Cul s 1 (rCul s 1) was investigated by immunoblotting, histamine release testing (HRT) and intradermal testing (IDT) in eight SE+ and eight control horses. Seven SE+ horses had rCul s 1-specific IgE, whereas only one control animal had IgE directed against this allergen. Furthermore, the HRT showed rCul s 1 induced basophil degranulation in samples from seven of eight SE+ horses but in none of the control animals. rCul s 1 also induced immediate ...Continue Reading

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Citations

Apr 9, 2013·Veterinary Immunology and Immunopathology·Nathalie M A van der MeideEdwin Tijhaar
Jun 21, 2014·PeerJ·Christopher J Lehiy, Barbara S Drolet
Apr 8, 2014·Mediators of Inflammation·Ji-Fu WeiShaoheng He
Aug 19, 2015·Allergy·R S MuellerE Marti
Aug 22, 2012·Veterinary Immunology and Immunopathology·Nathalie M A van der MeideEdwin Tijhaar
May 12, 2012·Veterinary Immunology and Immunopathology·A SchaffartzikC Rhyner
May 26, 2015·Veterinary Immunology and Immunopathology·Sigridur JonsdottirSigurbjorg Torsteinsdottir
Apr 12, 2014·Medical and Veterinary Entomology·A UrbanekW Gi Łka
Nov 6, 2018·Acta Veterinaria Scandinavica·Sigurbjörg TorsteinsdottirEliane Marti
Jun 22, 2018·Parasites & Vectors·Christopher J LehiyBarbara S Drolet
Jun 20, 2018·Veterinary Immunology and Immunopathology·Sigridur JonsdottirSigurbjorg Torsteinsdottir

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