Abstract
3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only a few KstDs have thus far been characterized biochemically and applied for the production of steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, and KstD3, were identified in Mycobacterium neoaurum DSM 1381, and they shared up to 99, 85 and 97% amino acid identity with previously reported KstDs, respectively. In this paper, KstDs from M. neoaurum DSM 1381 were investigated and exemplified their potential application for industrial steroid transformation. The recombinant KstD2 from Bacillus subtilis exhibited higher enzymatic activity when 4-androstene-3,17-dione (AD) and 22-hydroxy-23, 24-bisnorchol-4-ene-3-one (4HP) were used as the substrates, and resulted in specific activities of 22.40 and 19.19 U mg-1, respectively. However, the specific activities of recombinant KstD2 from Escherichia coli, recombinant KstD1 from B. subtilis and E. coli, and recombinant KstD3, also fed with AD and 4HP, had significantly lower specific activities. We achieved up to 99% bioconversion rate of 1,4-androstadiene-3,17-dione (ADD) from 8 g L-1 AD after 15 h of fermentation using E. coli transformant B...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Jun 6, 1991·Nature·C K StoverG F Hatfull
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1997·Steroids·S B Mahato, S Garai
May 2, 2000·Applied and Environmental Microbiology·R van Der GeizeL Dijkhuizen
Aug 10, 2000·Microbiology·T Parish, N G Stoker
Oct 9, 2002·Microbiology·Robert van der GeizeLubbert Dijkhuizen
Jan 14, 2005·Applied Microbiology and Biotechnology·M V DonovaI F Puntus
Dec 21, 2006·Nature Methods·Julia C van Kessel, Graham F Hatfull
Feb 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert Van der GeizeLindsay D Eltis
Mar 10, 2007·Prikladnaia biokhimiia i mikrobiologiia·M V Donova
Jul 13, 2007·Current Microbiology·Hoang Duc NguyenWolfgang Schumann
Jul 20, 2007·Molecular Microbiology·Sharon L KendallNeil G Stoker
Aug 9, 2007·Bioorganicheskaia khimiia·M A MolchanovaN E Voĭshvillo
Nov 23, 2007·The Biochemical Journal·Jan KnolRobert van der Geize
Feb 20, 2010·Microbiology·Sharon L KendallNeil G Stoker
Mar 10, 2010·Sub-cellular Biochemistry·Alice Vrielink
May 8, 2010·The Journal of Biological Chemistry·Carola DresenLindsay D Eltis
May 11, 2010·Applied and Environmental Microbiology·Wei WeiDong-Zhi Wei
Jun 4, 2010·PloS One·Nuria AndreuSiouxsie Wiles
Jun 15, 2010·Applied and Environmental Microbiology·Alessandro CascioferroRiccardo Manganelli
Jul 16, 2010·Microbiological Research·Laura Fernández de Las HerasOliver Drzyzga
Jan 12, 2012·Applied Microbiology and Biotechnology·Miao-Miao ChenDong-Zhi Wei
Feb 9, 2012·Microbial Biotechnology·J L GarcíaB Galán
May 9, 2012·Applied Microbiology and Biotechnology·Marina V Donova, Olga V Egorova
Jul 10, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Laura Fernández de las HerasJuana María Navarro Llorens
Jan 10, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Wenqing ZhangZhenghong Xu
Mar 12, 2013·The Journal of Steroid Biochemistry and Molecular Biology·E Yu BraginM V Donova
Oct 30, 2013·The Journal of Biological Chemistry·Ali RohmanBauke W Dijkstra
Jan 17, 2014·Microbial Cell Factories·Haiyong WangGuanghui Li
May 17, 2014·Metabolic Engineering·Kang YaoDong-Zhi Wei
Jan 13, 2015·Journal of Industrial Microbiology & Biotechnology·Rili XieMin Wang
Feb 18, 2016·Journal of Industrial Microbiology & Biotechnology·Minglong ShaoShangtian Yang
Feb 24, 2016·Scientific Reports·Li-Qin XuDong-Zhi Wei
Jul 6, 2016·Journal of Industrial Microbiology & Biotechnology·Yang LiuMin Wang
Nov 1, 2016·Journal of Applied Microbiology·M ShaoS Yang
Nov 3, 2016·Microbial Biotechnology·Beatriz GalánJosé L García
Mar 16, 2017·Microbial Cell Factories·Govinda GuevaraJuana María Navarro Llorens
Jun 22, 2017·Applied Microbiology and Biotechnology·Xiaojun WangYanhe Ma
Citations
Jul 11, 2019·Applied Microbiology and Biotechnology·Ruijie ZhangBaoguo Zhang
Jan 30, 2020·Microbial Cell Factories·Yang ZhangMin Wang
Jun 27, 2019·BMC Biotechnology·Eugeny Y BraginMarina V Donova
Apr 2, 2020·Microbial Cell Factories·Liang-Bin XiongDong-Zhi Wei
Sep 10, 2020·Plants·Rituraj BatthHenrik Toft Simonsen
Mar 5, 2021·Microbial Biotechnology·Carmen Felpeto-SanteroJosé Luis García
Apr 22, 2020·Bioresource Technology·Xiuling ZhouMin Wang
Apr 7, 2021·Biotechnology Advances·Ali Rohman, Bauke W Dijkstra
Aug 14, 2020·Journal of Agricultural and Food Chemistry·Jian-Mei LuoMin Wang