Identification, functional characterization, and developmental expression of two nonallelic parathyroid hormone (PTH)/PTH-related peptide receptor isoforms in Xenopus laevis (Daudin)

Endocrinology
C BergwitzH Jüppner

Abstract

Complementary DNAs encoding two nonallelic PTH/PTH-related peptide (PTHrP) receptor (PPR) isoforms, xPPR-A and xPPR-B, were isolated from a kidney complementary DNA library of the tetraploid African clawed frog Xenopus laevis. Both isoforms differ in their coding region by 19 amino acids, and lack the region corresponding to the mammalian exon E2. When expressed in mammalian COS-7 cells, both receptor isoforms bound radiolabeled PTH-(1-34) and PTHrP-(1-36) analogs with comparable affinity, and both unlabeled peptides equivalently stimulated the accumulation of cAMP. xPPR-A also mediated inositol phosphate turnover in COS cells and stimulated channel-mediated current changes in voltage clamp experiments after injection into oocytes. Using ribonuclease protection analysis, significant xPPR-A messenger RNA expression was first detected in neurula stage embryos, which subsequently increased approximately 30-fold during tadpole development. Expression reached a maximum at the metamorphotic climax, when isoform B also became detectable at significant levels, and subsequently declined in postmetamorphotic froglets. In the adult frog, xPPR-A was prominently expressed in lung, brain, small bowel, and skin, whereas isoform B was highest ...Continue Reading

Citations

Jul 10, 2012·BMC Evolutionary Biology·Pedro L C PinheiroAdelino V M Canário
Dec 3, 2010·BMC Evolutionary Biology·Pedro L C PinheiroAdelino V M Canário
Sep 12, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M L CancelaP A Price
Sep 10, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tao QiuXu Cao
Sep 15, 2005·American Journal of Physiology. Renal Physiology·Clint PerryIrina I Grichtchenko
Apr 13, 2019·Clinical Kidney Journal·Pablo A Ureña-TorresUNKNOWN ERA-EDTA CKD-MBD Working Group

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