Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines

Journal of Proteomics
Sanyuan TangYujuan Yuan

Abstract

Multidrug resistance (MDR) to anticancer drugs is a major obstacle to successful chemotherapy of tumors. Understanding the molecular basis to chemoresistance is likely to provide better treatment. Cell lines resistant to cis-diamminedichloroplatinum (CNE2/cDDP) were established from human nasopharyngeal carcinoma (NPC) cell lines CNE2. Comparative proteomics involving 2-dimensional gel electrophoresis (2-DE) and ESI-Q-TOF-MS were performed on protein extracted from CNE2 and CNE2/cDDP cell lines to screen drug resistance-related proteins. Keratin 1 (KRT1), cathepsin D (CTSD) and annexin a5 (ANXA5) were identified as three proteins showing higher expression in CNE2/cDDP compared to CNE2. Furthermore, suppression of KRT1 expression by siRNA resulted in decreased MDR in siRNA-CNE2/cDDP cells. And upregulation of KRT1 could result in increased of drug resistance in NPC cell lines. Taken together, KRT1 protein and its activity levels were higher in cDDP-resistant NPC cell lines compared to their parental cell lines. These data clearly linked KRT1 and cDDP resistance mechanisms. KRT1 could serve as a biomarker for chemotherapy sensitivity of NPC.

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Citations

Mar 13, 2014·Expert Review of Proteomics·Liang XiaoZhi-Qiang Xiao
Jul 18, 2015·International Journal of Molecular Sciences·Ze-Tan ChenXiao-Dong Zhu
Oct 15, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Boya PengMing-Zhong Sun
May 25, 2015·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Laura GattiPaola Perego
Sep 24, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lei WuXingen Zhu
Feb 15, 2018·International Journal of Molecular Sciences·Katarzyna Zabielska-KoczywąsRoman Lechowski
May 7, 2019·Expert Review of Proteomics·Zhefeng Xiao, Zhuchu Chen

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