PMID: 8601586Mar 1, 1996Paper

Identification, localization, and functional implications of an abundant nematode annexin

The Journal of Cell Biology
C E CreutzJ Redick

Abstract

Cultures of the nematode C. elegans were examined for the presence of calcium-dependent, phospholipid-binding proteins of the annexin class. A single protein of apparent mass on SDS-polyacrylamide gels of 32 kD was isolated from soluble extracts of nematode cultures on the basis of its ability to bind to phospholipids in a calcium-dependent manner. After verification of the protein as an annexin by peptide sequencing, an antiserum to the protein was prepared and used to isolate a corresponding cDNA from an expression library in phage lambda gt11. The encoded protein, herein referred to as the nex-1 annexin, has a mass of 35 kD and is 36-42% identical in sequence to 10 known mammalian annexins. Several unique modifications were found in the portions of the sequence corresponding to calcium-binding sites. Possible phosphorylation sites in the NH2-terminal domain of the nematode annexin correspond to those of mammalian annexins. The gene for this annexin (nex-1) was physically mapped to chromosome III in the vicinity of the dpy-17 genetic marker. Two other annexin genes (nex-2 and nex-3) were also identified in chromosome III sequences reported by the nematode genomic sequencing project (Sulston, J., Z. Du, K. Thomas, R. Wilson, L...Continue Reading

References

Nov 6, 1992·Science·C E Creutz
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May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A L BurnsH B Pollard
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Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R R ZwaalR H Plasterk

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Citations

Sep 28, 1998·Biochimica Et Biophysica Acta·A J Avila-SakarR H Kretsinger
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Jun 12, 2003·Genome Research·Steven A FarberMarnie E Halpern
Jun 3, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·F Russo-Marie
Apr 27, 2010·Experimental Parasitology·Cibele Aparecida TararamLuciana Cezar de Cerqueira Leite
Apr 7, 2004·Development, Growth & Differentiation·Aruni PereraJohn J Robinson
Nov 26, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Andreas HofmannMalcolm K Jones
May 18, 1999·Biochemical and Biophysical Research Communications·Y WenG Sachs
Apr 12, 2003·Developmental Cell·Swathi ArurDavid K Han
Oct 11, 1996·Biochimica Et Biophysica Acta·H KawasakiR H Kretsinger
Oct 11, 1996·Biochimica Et Biophysica Acta·T DuboisF Russo-Marie
Jun 27, 1997·Biochimica Et Biophysica Acta·V Gerke, S E Moss
Sep 10, 2004·Developmental Biology·Ken-Ichi KariyaTohru Kataoka
Mar 15, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sandeep SaxenaMohammed M Idris
Aug 15, 2002·Development, Growth & Differentiation·Janice Mayne, John J Robinson
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C PaupardD H Hall
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Mar 2, 2002·Traffic·Hanna Fares, Barth Grant

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