Identification of 11-dehydro-TXB2 as a suitable parameter for monitoring thromboxane production in the human

Prostaglandins
P WestlundA Nordenström

Abstract

In order to identify suitable parameters for measurement of thromboxane production in vivo, the metabolism of TXB2 was studied in the human. [3H8]-TXB2 was given intravenously to a healthy human volunteer. Blood samples were collected for 50 min after the injection, and urine was collected for 24 hours. The urinary and blood metabolic profiles were visualized by the use of two-dimensional TLC and autoradiography. Identification of metabolites was achieved with GC/MS and in some cases by cochromatography with reference compounds in TLC and GC. In blood, unmetabolized TXB2 was the dominating compound during the first 30 min. Three less polar metabolites appeared, two of which were identified as 11-dehydro-TXB2 and 11,15-didehydro-13,14-dihydro-TXB2, respectively. The third compound was tentatively identified as 15-dehydro-13,14-dihydro-TXB2. Since 11-dehydro-TXB2 was one of the major metabolites in blood as well as urine, it was deemed suitable as target for measurement of thromboxane production in vivo. The advantages of 11-dehydro-TXB2 over its parent compound, TXB2, were demonstrated in experiments where unlabeled TXB2 was injected i.v. to a human volunteer, and the blood and urinary levels of both compounds were then followed...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·B SamuelssonC Malmsten
Apr 1, 1977·Prostaglandins·H Kindahl
Jan 1, 1975·Annual Review of Biochemistry·B SamuelssonS Hammarström
May 1, 1975·Angewandte Chemie·W Bartmann
Nov 1, 1976·Acta Physiologica Scandinavica·J SvenssonB Samuelsson
Feb 1, 1988·Drugs·I A Reilly, G A FitzGerald
Jan 1, 1986·Progress in Lipid Research·E Granström
Feb 1, 1986·The Journal of Clinical Investigation·C PatronoG A FitzGerald
May 1, 1981·Prostaglandins·H G MorrisF T Shepperdson
Jan 1, 1982·Methods in Enzymology·R L MaasL J Roberts

❮ Previous
Next ❯

Citations

Feb 1, 1995·Clinical Autonomic Research : Official Journal of the Clinical Autonomic Research Society·M Kotamäki
Apr 24, 1987·Biochimica Et Biophysica Acta·G CiabattoniC Patrono
May 9, 1988·FEBS Letters·M Reinke, K Brune
Oct 1, 1988·Journal of the American College of Cardiology·B Q ZhuW W Parmley
Jul 14, 1998·Prostaglandins & Other Lipid Mediators·M DadaianP Westlund
Mar 12, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Lilian Rumi TsurutaMichinao Mizugaki
Apr 23, 2002·Acta Obstetricia Et Gynecologica Scandinavica·Masatoshi HayashiTakeyoshi Ohkura
Oct 1, 1989·Journal of the American College of Nutrition·J T JuddJ Dupont
Jan 1, 1993·Acta Physiologica Scandinavica·H I ChenW C Chang
May 11, 1991·The American Review of Respiratory Disease·W R Henderson
Oct 14, 2000·Journal of Endovascular Therapy : an Official Journal of the International Society of Endovascular Specialists·J R BoyleM M Thompson
Jun 1, 1990·Biomedical & Environmental Mass Spectrometry·M IshibashiN Harima
May 29, 2004·Prostaglandins & Other Lipid Mediators·Naoto SuzukiJunichi Goto
Feb 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Y TannirandornM G Elder
Jul 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T NagaseH Orimo
Apr 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M H SullivanM G Elder
Sep 15, 1988·The New England Journal of Medicine·J A OatesL J Roberts
Aug 1, 1988·Biomedical & Environmental Mass Spectrometry·M IshibashiS Krolik
Dec 3, 2016·American Journal of Veterinary Research·Brittany Heggem-PerryJason Tarricone
Apr 30, 1990·Biochemical and Biophysical Research Communications·T NagaseH Orimo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.