Identification of 14-3-3zeta as an EGF receptor interacting protein

FEBS Letters
Morten P OksvoldWallace Y Langdon

Abstract

The 14-3-3 proteins are known to interact with a number of proteins involved in the regulation of cell signaling. Here, we describe an association of 14-3-3zeta with the epidermal growth factor receptor (EGFR) that is rapidly induced by EGF. The 1028-EGFR truncated mutant which lacks the cytoplasmic tail from amino acids 1029-1186 identified the binding site for 14-3-3 to be between amino acid 1028 and the receptor carboxyl terminus. Mutational deletion of serine residues 1046, 1047, 1057 and 1142 did not inhibit EGF-induced 14-3-3 association with the receptor. Immunofluorescence microscopy indicated an EGF-induced co-localization of EGFR and HA-14-3-3zeta along the plasma membrane. Our finding adds to the growing complexity of EGF receptor signaling and indicates a role for 14-3-3 proteins in EGF receptor signaling or regulation.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R W Donaldson, S Cohen
Jun 17, 1998·Molecular Neurobiology·E M Skoulakis, R L Davis
Feb 1, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M P OksvoldH S Huitfeldt
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Aug 5, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Carpenter
Jul 12, 2001·Yeast·M J van HemertH Y Steensma
Oct 19, 2001·Oncogene·G TzivionJ Zhu
Nov 16, 2001·The Journal of Biological Chemistry·Guri Tzivion, Joseph Avruch
Jul 17, 2003·The Journal of Biological Chemistry·Joanna M WoodcockAngel F Lopez

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May 9, 2007·Reviews in Endocrine & Metabolic Disorders·R I NicholsonJ M W Gee
Dec 24, 2011·Cancer Gene Therapy·X YangH Lin
Apr 20, 2006·Clinical and Experimental Pharmacology & Physiology·Ming HeZhijun Luo
Jan 5, 2006·Respiratory Research·Alasdair J EdgarJulia M Polak
Jul 23, 2014·PloS One·Pasquale SimeoneSaverio Alberti
Jan 30, 2013·Brain Tumor Pathology·Wei-Dong CaoTakashi Tamiya
Apr 20, 2012·Expert Opinion on Therapeutic Targets·Ajay MattaRanju Ralhan
Mar 5, 2008·Experimental Parasitology·Mar Siles-LucasBruno Gottstein
Nov 2, 2005·Journal of Neuroscience Methods·Jun-ichi SatohTakashi Yamamura
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Feb 7, 2019·Apoptosis : an International Journal on Programmed Cell Death·Timothy HansenShermali Gunawardena
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