Identification of 3 beta-hydroxysteroid dehydrogenase as a novel target of steroid cell autoantibodies: association of autoantibodies with endocrine autoimmune disease

The Journal of Clinical Endocrinology and Metabolism
S ArifM Peakman

Abstract

Autoantibodies directed against steroid hormone-producing cells (SCA) detectable by immunofluorescence are typically found in a small proportion of patients with premature ovarian failure (POF) as well as in other endocrine autoimmune diseases. The SCA pattern stains cells in the outer zones of the adrenal cortex, ovary, and testis. To identify the molecular target of SCA, an adrenal complementary DNA expression library was screened using SCA-positive serum, and the steroid enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) was identified. Only 1 of 48 (2%) patients with idiopathic POF, not pre-selected for the presence of other autoimmune diseases, had SCA by immunofluorescence, whereas 10 of 48 (21%) had anti-3 beta HSD autoantibodies detectable by immunoblot using recombinant human enzyme compared with 6 of 115 (5%) control subjects (P = 0.002). Absorption of SCA-positive serum with recombinant human 3 beta HSD abolished the immunofluorescence pattern. We also examined the prevalence of anti-3 beta HSD autoantibodies in other endocrine autoimmune diseases. Two of 112 (2%) diabetic patients, but none of the thyroid or Addisonian patients, had SCA by immunofluorescence. Twenty-six (23%) diabetic subjects (P < 0.001 vs. co...Continue Reading

Citations

Jun 4, 2010·Journal of Assisted Reproduction and Genetics·Eusebio S Pires
Jan 12, 2002·The American Journal of Pathology·Harini BagavantKenneth S K Tung
Oct 19, 2011·Clinical & Developmental Immunology·Kadri Haller-KikkataloRaivo Uibo
Jun 14, 2005·Journal of Reproductive Immunology·Radhika L KelkarTarala D Nandedkar
Apr 14, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Patricia Monnier-BarbarinoMarie Christine Béné
Dec 14, 2011·Autoimmunity Reviews·Jana Petríková, Ivica Lazúrová
Jul 3, 2002·Endocrinology and Metabolism Clinics of North America·Alberto FalorniFausto Santeusanio
Mar 31, 2009·Endocrinology and Metabolism Clinics of North America·Eystein S Husebye, Kristian Løvås

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