Identification of a 16SrII-E Phytoplasma in Calendula arvensis, Solanum nigrum, and Chenopodium spp

Plant Disease
G ToluA Bertaccini

Abstract

Epidemiological surveys were performed in Northern Sardinia (Italy) in a 10-year-old vineyard affected by "Bois noir" disease. Samples collected between May and October 2003 from chlorotic and stunted weeds belonging to 14 different taxonomic groups were indexed molecularly for detection of phytoplasmas. Nested polymerase chain reaction (PCR) assays using primers specific for the phytoplasma 16SrDNA gene showed three of six Calendula arvensis, one of two Solanum nigrum, and one of seven Chenopodium spp. assayed positive. Restriction fragment length polymorphism analyses and sequencing of amplified 16SrDNA fragments identified a putative phytoplasma in the ribosomal subgroup 16SrII-E. Further characterization of the rps3 gene, coding a ribosomal protein, confirmed the identification. However, the weeds and leafhop-per species collected in the vineyard tested negative by PCR assays for the Stolbur phytoplasma, the causal agent of "Bois noir". This is the first report of a phytoplasma of the 16SrII-E subgroup infecting C. arvensis, S. nigrum, and Chenopodium spp.

References

Mar 7, 2003·Canadian Journal of Microbiology·Norma Elena Leyva-LópezJuan Pablo Martínez-Soriano
Jul 29, 2004·International Journal of Systematic and Evolutionary Microbiology·UNKNOWN IRPCM Phytoplasma/Spiroplasma Working Team--Phytoplasma Taxonomy Group
May 1, 2005·Plant Disease·M A Al-SakeitiM L Deadman

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Citations

May 7, 2020·Folia Microbiologica·Sara Gharouni-KardaniAssunta Bertaccini
Aug 1, 2009·Plant Disease·A GajardoA Bertaccini
Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Marie-Agnès Jacques
Oct 1, 2006·Plant Disease·B Duduk, A Bertaccini
Dec 31, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Marie-Agnès Jacques
Dec 31, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Marie-Agnès Jacques

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