Identification of a 66 kDa Haemonchus contortus excretory/secretory antigen that inhibits host monocytes

Veterinary Parasitology
D K RathoreParitosh Joshi

Abstract

A 66 kDa adult Haemonchus contortus excretory/secretory (E/S) antigen was identified in Western blot by reaction with sera from the infected goats. The protein was purified from the adult worm extract and E/S products by anion exchange and ConA-Sepharose chromatography. The purified protein inhibited monocyte function in vitro as judged by decreased production of hydrogen peroxide and nitric oxide in the culture medium. The protein also caused proliferation of peripheral blood mononuclear cells. The absence of protein in the free living L3 larvae suggests that the expression of this protein coincides with the adaptation to the parasitic life. A correlation of antibody titre and worm burden was observed in the infected goats with higher antibody levels in high worm burdened animals. Anti-protein antibody caused loss of adult worm motility in vitro resulting in the death of the parasite. The fact that the protein is recognized by the host together with in vitro killing of adult parasites by antibodies makes this protein a promising candidate for vaccination trial.

References

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Citations

Dec 17, 2008·Parasitology·M J G JohnstonD M McKay
May 1, 2007·Pakistan Journal of Biological Sciences : PJBS·M M El-BahyH A Shalaby
May 12, 2009·Molecular and Biochemical Parasitology·S NareshaP Joshi
Dec 22, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Irfan-Ur-Rauf TakFayaz Ahmad
Feb 23, 2019·Parasitology Research·Jinglong ChenXiaojing Yang
Dec 15, 2018·Parasite Immunology·Parvathy RajanParitosh Joshi

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Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

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