Identification of a candidate stem cell in human gallbladder

Stem Cell Research
Rohan ManoharEric Lagasse

Abstract

There are currently no reports of identification of stem cells in human gallbladder. The differences between human gallbladder and intrahepatic bile duct (IHBD) cells have also not been explored. The goals of this study were to evaluate if human fetal gallbladder contains a candidate stem cell population and if fetal gallbladder cells are distinct from fetal IHBD cells. We found that EpCAM+CD44+CD13+ cells represent the cell population most enriched for clonal self-renewal from primary gallbladder. Primary EpCAM+CD44+CD13+ cells gave rise to EpCAM+CD44+CD13+ and EpCAM+CD44+CD13- cells in vitro, and gallbladder cells expanded in vitro exhibited short-term engraftment in vivo. Last, we found that CD13, CD227, CD66, CD26 and CD49b were differentially expressed between gallbladder and IHBD cells cultured in vitro indicating clear phenotypic differences between the two cell populations. Microarray analyses of expanded cultures confirmed that both cell types have unique transcriptional profiles with predicted functional differences in lipid, carbohydrate, nucleic acid and drug metabolism. In conclusion, we have isolated a distinct clonogenic population of epithelial cells from primary human fetal gallbladder with stem cell characteri...Continue Reading

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Citations

Jun 17, 2015·Stem Cell Research·Feorillo H GalivoMarkus Grompe
Jul 22, 2019·Veterinary Research·Dvir MintzNahum Y Shpigel
Mar 20, 2021·Molecular Therapy. Methods & Clinical Development·Kang Cui, Xinyan Bian
Feb 28, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Junya ShiotaNataliya Razumilava

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