Identification of a candidate therapeutic antibody for treatment of canine B-cell lymphoma

Veterinary Immunology and Immunopathology
Sarah M RueMarc Nasoff

Abstract

B-cell lymphoma is one of the most frequently observed non-cutaneous neoplasms in dogs. For both human and canine BCL, the standard of care treatment typically involves a combination chemotherapy, e.g. "CHOP" therapy. Treatment for human lymphoma greatly benefited from the addition of anti-CD20 targeted biological therapeutics to these chemotherapy protocols; this type of therapeutic has not been available to the veterinary oncologist. Here, we describe the generation and characterization of a rituximab-like anti-CD20 antibody intended as a candidate treatment for canine B-cell lymphoma. A panel of anti-canine CD20 monoclonal antibodies was generated using a mouse hybridoma approach. Mouse monoclonal antibody 1E4 was selected for construction of a canine chimeric molecule based on its rank ordering in a flow cytometry-based affinity assay. 1E4 binds to approximately the same location in the extracellular domain of CD20 as rituximab, and 1E4-based chimeric antibodies co-stain canine B cells in flow cytometric analysis of canine leukocytes using an anti-canine CD21 antibody. We show that two of the four reported canine IgG subclasses (cIgGB and cIgGC) can bind to canine CD16a, a receptor involved in antibody-dependent cellular cy...Continue Reading

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Citations

May 12, 2017·JFMS Open Reports·Jessica F RomineMarc S Elie
Feb 21, 2018·Frontiers in Immunology·Hans Klingemann
Sep 17, 2020·Nature Reviews. Cancer·Amy K LeBlanc, Christina N Mazcko
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Aug 21, 2020·The Veterinary Clinics of North America. Small Animal Practice·Robert Goggs
Apr 20, 2021·Frontiers in Veterinary Science·Fulvio Riondato, Stefano Comazzi
Jun 14, 2021·Journal of Comparative Pathology·Yuki MorinagaTakuya Mizuno
Aug 24, 2021·Frontiers in Immunology·Hans Klingemann

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