Identification of a CD4+CD25+ T cell subset committed in vivo to suppress antigen-specific T cell responses without additional stimulation

European Journal of Immunology
Esther N M Nolte-'t HoenMarca H M Wauben

Abstract

Naturally occurring CD4+ regulatory T cells can be identified on the basis of expression of CD25 and suppression of T cell responses in vitro after TCR triggering. Here, we demonstrate that a CD134+ subset of CD4+CD25+ T cells in naive rats suppresses antigen-specific T cell responses in vitro without additional TCR stimulation. In contrast, CD4+CD25+CD134- regulatory T cells and total CD4+CD25+ regulatory T cells have suppressive activity only during simultaneous activation of responder and regulatory T cells or after in vitro pre-activation. Furthermore CD4+CD25+CD134+ T cells have a more activated phenotype than CD4+CD25+CD134- T cells, as based on the expression of CD62L, CD45RC, and MHC class II. We propose that the CD134+ regulatory T cells contain an in vivo activated and highly suppressive regulatory T cell subset. CD4+CD25+CD134+ T cells can be found in several compartments of the immune system, including spleen, lymph nodes, and blood. Interestingly though, the relative amounts of these cells within the CD4+ population and their CD134 expression levels are highest in mucosa-draining lymph nodes and lowest in blood. This suggests that the presence of CD4+CD25+CD134+ T cells indicates sites of active immune suppression.

References

Jan 1, 1984·The Journal of Clinical Investigation·J HoloshitzI R Cohen
Mar 2, 1999·The Journal of Experimental Medicine·B Seddon, D Mason
Jul 10, 1999·Advances in Immunology·A M Faria, H L Weiner
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M Thornton, E M Shevach
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K M Thorstenson, A Khoruts
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Baecher-AllanD A Hafler
Dec 12, 2001·Nature Immunology·Marc A GavinAlexander Rudensky
Jun 6, 2002·Immunology·Kajsa WingElisabeth Suri-Payer
Jul 3, 2002·Nature Reviews. Immunology·Ethan M Shevach
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joachim LehmannAlf Hamann
Mar 5, 2003·European Journal of Immunology·Chia-Huey Lin, Thomas Hünig
Mar 27, 2003·Nature Reviews. Immunology·Matthias G von Herrath, Leonard C Harrison
Jun 17, 2003·Immunology·Arne N AkbarMilica Vukmanovic-Stejic
Jul 23, 2003·The Journal of Experimental Medicine·Lucy S K WalkerAbul K Abbas
Aug 27, 2003·The Journal of Experimental Medicine·Sylvain FissonBenoît L Salomon

❮ Previous
Next ❯

Citations

Nov 15, 2008·Cancer Immunology, Immunotherapy : CII·Christine GalustianAngus G Dalgleish
Jul 15, 2006·Springer Seminars in Immunopathology·Shelly J Robertson, Kim J Hasenkrug
Aug 12, 2005·Current Opinion in Rheumatology·Eva C KoffemanSalvatore Albani
Jan 13, 2009·Blood·Michelle M CollazoWilliam G Kerr
May 19, 2005·Arthritis Research & Therapy·Elmieke P J BootMarca H M Wauben
Dec 21, 2012·Annals of the New York Academy of Sciences·Miriam C SouroujonSara Fuchs
Dec 21, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Revital ArichaSara Fuchs
Jun 1, 2020·Scandinavian Journal of Immunology·Na XiaoZonghong Shao
Apr 29, 2016·Clinical and Experimental Immunology·A L Rodríguez-PereaP A Velilla
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shelly J RobertsonKim J Hasenkrug
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Revital ArichaMiriam C Souroujon
May 13, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R A BascomL J West
Oct 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kritika KachapatiWilliam M Ridgway

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.