Identification of a cell wall-associated subtilisin-like serine protease involved in the pathogenesis of Streptococcus suis serotype 2

Microbial Pathogenesis
Qiaoyun HuMeilin Jin

Abstract

Streptococcus suis is an important swine and human pathogen, and also an emerging zoonotic agent. A surface-associated subtilisin-like serine protease (SspA) of S. suis was identified by screening a genomic expression library as fragments of this protein reacted most strongly with convalescent-phase pig sera. The sspA gene is present in 29 of 33 S. suis serotypes reference strains and is expressed on the surface of S. suis. Relative real-time quantitative PCR assay demonstrated that sspA mRNA expression in vivo was several thousand fold of that in vitro. A sspA(-) mutant was generated from a S. suis serotype 2 strain SC19 by allelic exchange. The mutant was not different from the wild type strain in subcellular structures and in hemolytic phenotype. However, the virulence of the sspA(-) mutant was markedly lower than the wild type in pigs as demonstrated in experimental infections. These data indicated that the surface-associated protein SspA is a conserved virulence factor of S. suis and is involved in the pathogenesis of S. suis.

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Citations

Jul 12, 2011·Applied and Environmental Microbiology·Laetitia BonifaitDaniel Grenier
Mar 15, 2011·Journal of Bacteriology·Pan HuMeilin Jin
Apr 2, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Quanju ZhaoJianping Xie
Apr 19, 2011·Nihon saikingaku zasshi. Japanese journal of bacteriology·Daisuke Takamatsu
Oct 4, 2015·Cellular Microbiology·Timothy C BarnettMark J Walker
Dec 8, 2010·Microbial Pathogenesis·Tatsuya NakayamaKazunori Oishi
Aug 27, 2010·FEMS Microbiology Letters·Laetitia BonifaitDaniel Grenier
Sep 27, 2020·Veterinary Sciences·Chengkun ZhengManMan Cao

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