Nov 1, 1977

Identification of a chromosome that controls malignancy in Chinese hamster cells

Journal of Cellular Physiology
N Bloch-Shtacher, L Sachs

Abstract

A chromosome that controls malignancy in Chinese hamster cells has been identified by analysis of the Giemsa banding pattern of a malignant cell line transformed by simian virus 40 (SV40), non-malignant revertants from this line, segregants from the revertants that were again malignant and a cell line transformed by methylcholanthrene. The malignant cell line transformed by SV40 was near diploid and had gained additional material of chromosome 3. Revertants with a suppression of malignancy and malignant revertants from which they were derived. Malignancy of these cells was associated with the ability to form colonies in agar. Cells of a line transformed by methylcholanthrene were malignant, formed almost no colonies in agar and the only chromosome change from the normal diploid chromosome banding complement was the addition of a long arm of chromosome 3. The results indicate that chromosome 3 carriers gene(s) that control malignancy in Chinese hamster cells in cell lines transformed by a viral or a chemical carcinogen and that malignancy was induced in both cell types by an increase of these genes.

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References

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Citations

Mentioned in this Paper

20-Methylcholanthrene
Chinese Hamster
Chromosome Banding
Agar
Methylcholanthrene
Simian virus 40
Primary Malignant Neoplasm
Visual Suppression
Malignant Paraganglionic Neoplasm
Tumor Cells, Malignant

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