Identification of a common gene expression signature associated with immature clonal mesenchymal cell populations derived from bone marrow and dental tissues

Stem Cells and Development
Danijela MenicaninS Gronthos

Abstract

Mesenchymal stem/stromal cell-like populations derived from adult bone marrow (BMSC), dental pulp (DPSC), and periodontal ligament (PDLSC) have the ability to differentiate into cells of mesenchymal and non-mesenchymal tissues in vitro and in vivo. However, culture-expanded MSC-like populations are a heterogeneous mix of stem/committed progenitor cells that exhibit altered growth and developmental potentials. In the present study we isolated and characterized clonal populations of BMSCs, DPSCs, and PDLSCs to identify potential biomarkers associated with long-lived multipotential stem cells. Microarray analysis was used to compare the global gene expression profiles of high growth/multipotential clones with low growth potential cell clones derived from 3 stromal tissues. Cross-comparison analyses of genes expressed by high growth/multipotential clones derived from bone marrow, dental pulp, and periodontal ligament identified 24 genes that are differentially up-regulated in all tissues. Notably, the transcription factors, E2F2, PTTG1, TWIST-1, and transcriptional cofactor, LDB2, each with critical roles in cell growth and survival, were highly expressed in all stem cell populations examined. These findings provide a model system ...Continue Reading

References

Oct 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S S MurrayE J Murray
Mar 15, 1995·Genes & Development·Z F Chen, R R Behringer
Apr 1, 1997·Molecular Endocrinology·L Pei, S Melmed
Sep 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S A KuznetsovP G Robey
Feb 18, 1999·The Journal of Clinical Endocrinology and Metabolism·X ZhangS Melmed
Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 26, 1999·Journal of Cellular Biochemistry·M S LeeC A Glackin
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Feb 22, 2001·Science·J C VenterX Zhu
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
May 24, 2001·Cell·P V JallepalliC Lengauer
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Jeffrey M Trimarchi, Jacqueline A Lees
May 22, 2002·Biochimica Et Biophysica Acta·James DeGregori
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joseph S Beckman
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Oct 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jaejoon WonTae Kook Kim
Dec 11, 2002·Current Opinion in Cell Biology·Olivier Stevaux, Nicholas J Dyson
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Stan GronthosSongtao Shi
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
May 3, 2003·Cancer Cell·Hugh Cam, Brian David Dynlacht
May 10, 2003·European Journal of Orthodontics·David P C RiceIrma Thesleff
Feb 5, 2004·The Breast : Official Journal of the European Society of Mastology·Christine SolbachManfred Kaufmann
Mar 20, 2004·Developmental Cell·Henry M Kronenberg
Mar 20, 2004·Developmental Cell·Peter BialekGerard Karsenty
Mar 17, 2005·The Journal of Clinical Endocrinology and Metabolism·Shaw-Jenq TsaiLih-Yuh C Wing
Feb 28, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jeannette ConnerneyDouglas B Spicer
Jul 13, 2007·American Journal of Physiology. Cell Physiology·Tami RubinekShlomo Melmed
May 2, 2008·Journal of Cellular Physiology·Uri David AkaviaDafna Benayahu

❮ Previous
Next ❯

Citations

Aug 14, 2013·PloS One·Enrica EleuterioStefania Angelucci
Oct 23, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Peter StankoCestmir Altaner
Jan 2, 2014·Stem Cells and Development·Naohisa WadaAkifumi Akamine
Jul 28, 2012·Dental Clinics of North America·Harold E GoodisSami M A Chogle
May 11, 2011·Cytotherapy·Jorge S Burns, Moustapha Kassem
Apr 18, 2012·Periodontology 2000·Kim HynesP Mark Bartold
Jan 5, 2011·Journal of Cellular and Molecular Medicine·Javier CatónThimios A Mitsiadis
Oct 9, 2015·Journal of Hematology & Oncology·Jacqueline E NollAndrew Cw Zannettino
Dec 19, 2012·Archives of Oral Biology·Alejandro Iglesias-LinaresAsunción Mendoza-Mendoza
Jun 22, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Lina GölzDominik Kraus
May 12, 2016·Journal of Cellular Biochemistry·Jia NgStan Gronthos
Jun 17, 2011·Advances in Dental Research·Y HarichaneA Poliard
Jun 15, 2018·International Journal of Molecular Sciences·Ann-Kathrin SchubertMichael Sittinger
Jan 10, 2019·Journal of Clinical Periodontology·Mark BartoldSaso Ivanovski
Oct 12, 2019·International Journal of Molecular Sciences·Oriana TrubianiJacopo Pizzicannella
Aug 5, 2017·Stem Cells International·Adriana Di BenedettoGiorgio Mori
Apr 8, 2021·Scientific Reports·Tsugumi UedaShinya Murakami
May 29, 2021·Stem Cells International·Jingqiu ChenJun Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
gene array
column chromatography
chips
PCR

Software Mentioned

Lumi package
GeneSpring GX
lumi
R

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Masako MiuraS Shi
Proceedings of the National Academy of Sciences of the United States of America
S GronthosS Shi
© 2021 Meta ULC. All rights reserved