PMID: 9431986Feb 7, 1998Paper

Identification of a common structural motif in the disordered N-terminal region of bacterial flagellins--evidence for a new class of fibril-forming peptides

European Journal of Biochemistry
E HakalehtoJ Finne

Abstract

Flagellin proteins lacking the N- or C-terminus form polymers of reduced filament stability and straight morphology, in contrast to the coiled native flagella. In the present study, the N-terminal amino acid sequence of flagellins of the anaerobic beer spoilage bacteria Pectinatus cerevisiiphilus and Pectinatus frisingiensis as well as Enterobacter aerogenes and Pseudomonas sp. were determined. Sequence similarity was revealed between these and the N-termini of all known eubacterial flagellins. Synthetic peptides corresponding to the first 15 amino acid residues of the flagellins of Pectinatus, Campylobacter jejuni, E. aerogenes or Proteus mirabilis flagellins had a spontaneous tendency under physiological conditions to form 4-6 nm broad, 1-2 microm long fibrillar structures that had a tendency to form clusters. In contrast, the Pectinatus peptide missing residues 1-3 did not form fibrils. The peptide missing residues 13-15 formed fibrils less easily, and the peptide missing residues 11-15 formed fibrils almost without clustering. In electron micrographs, the fibrillisation of the bacterial flagellar peptides resembled that of beta-amyloid and prion peptides. 1H-NMR and infrared spectroscopy studies with homology analysis indic...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S S Zimmerman, H A Scheraga
Oct 25, 1975·Journal of Molecular Biology·A D McLachlanL B Smillie
Jun 1, 1992·Journal of Clinical Microbiology·W JiangP D Gorevic
Dec 1, 1992·Biophysical Journal·F VondervisztK Namba
Aug 5, 1992·Journal of Molecular Biology·F VondervisztS Aizawa
Mar 5, 1991·Journal of Molecular Biology·C HilbichK Beyreuther
Aug 1, 1990·Biochimie·J Garnier
Sep 5, 1989·Journal of Molecular Biology·F VondervisztK Namba
Nov 3, 1988·Nature·T G Oas, P S Kim
Jul 1, 1988·Journal of Bacteriology·G Kuwajima
May 5, 1988·Journal of Molecular Biology·H J DysonR A Lerner
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A KirschnerD J Selkoe
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A KirschnerD J Selkoe
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Aug 30, 1985·Science·G D RoseM H Zehfus
Dec 20, 1985·Journal of Molecular Biology·L N Wei, T M Joys
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G G GlennerJ D Termine
Dec 15, 1983·European Journal of Biochemistry·A PardiK Wüthrich
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F TagliaviniG Forloni
Jan 28, 1994·Biochemical and Biophysical Research Communications·Y HiroseS Kohsaka
Apr 8, 1993·Nature·G ForloniF Tagliavini
May 14, 1993·Biochemical and Biophysical Research Communications·J A JarvisM C Wilce
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y Mimori-KiyosueK Namba

❮ Previous
Next ❯

Citations

Aug 18, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Elias HakalehtoHeikki Paakkanen
Sep 23, 2008·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Elias HakalehtoHeikki Paakkanen
Apr 17, 2007·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Elias HakalehtoAnneli Heitto
Aug 31, 2018·The Journal of Experimental Medicine·Paramita ChakrabartyTodd E Golde
Nov 6, 2012·Journal of Proteome Research·T Yu SamginaA T Lebedev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Related Papers

Pathophysiology : the Official Journal of the International Society for Pathophysiology
Elias HakalehtoAnneli Heitto
Pathophysiology : the Official Journal of the International Society for Pathophysiology
Elias Hakalehto
Current Treatment Options in Gastroenterology
Virmeet V. Singh, Phillip P. Toskes
© 2021 Meta ULC. All rights reserved