Identification of a conserved archaeal RNA polymerase subunit contacted by the basal transcription factor TFB

The Journal of Biological Chemistry
C P MagillS D Bell

Abstract

Archaea possess two general transcription factors that are required to recruit RNA polymerase (RNAP) to promoters in vitro. These are TBP, the TATA-box-binding protein and TFB, the archaeal homologue of TFIIB. Thus, the archaeal and eucaryal transcription machineries are fundamentally related. In both RNAP II and archaeal transcription systems, direct contacts between TFB/TFIIB and the RNAP have been demonstrated to mediate recruitment of the polymerase to the promoter. However the subunit(s) directly contacted by these factors has not been identified. Using systematic yeast two-hybrid and biochemical analyses we have identified an interaction between the N-terminal domain of TFB and an evolutionarily conserved subunit of the RNA polymerase, RpoK. Intriguingly, homologues of RpoK are found in all three nuclear RNA polymerases (Rpb6) and also in the bacterial RNA polymerase (omega-subunit).

References

Sep 14, 1995·Nature·D B NikolovS K Burley
Oct 1, 1993·Molecular and Cellular Biology·S MalikR G Roeder
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A BarberisM Ptashne
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Buratowski, H Zhou
Feb 1, 1996·Nature Structural Biology·W ZhuR A Scott
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·P F KosaP B Sigler
May 15, 1997·The EMBO Journal·S A QureshiS P Jackson
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·S D BellS P Jackson
Jul 10, 1999·Journal of Bacteriology·T J DarcyJ N Reeve
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·S D BellS P Jackson
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O LittlefieldP B Sigler
Jan 5, 2000·The EMBO Journal·F T Tsai, P B Sigler
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·L MinakhinK Severinov
Apr 3, 2001·Current Opinion in Microbiology·S D Bell, S P Jackson

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Citations

Dec 17, 2002·Molecular and Cellular Biology·Magali SiautChristine Conesa
Mar 17, 2004·Molecular and Cellular Biology·Thomas C TubonWinship Herr
Mar 12, 2011·Biology Direct·Jeannette Marrero CotoBettina Siebers
May 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Karim-Jean ArmachePatrick Cramer
Jun 16, 2004·Current Opinion in Genetics & Development·Mohamed Ouhammouch
Apr 16, 2003·Molecular Microbiology·John N Reeve
Aug 16, 2006·Trends in Microbiology·Renjith Mathew, Dipankar Chatterji
Oct 27, 2005·Current Opinion in Microbiology·Michael S Bartlett
Feb 18, 2011·Transcription·Sacha De CarloAndreas Hoenger
Nov 1, 2002·Molecular Cell·Finn Werner, Robert O J Weinzierl
Nov 18, 2003·The Journal of Biological Chemistry·Michael S BartlettE Peter Geiduschek
Jun 4, 2002·The Journal of Biological Chemistry·Arie B BrinkmanJohn van der Oost
Sep 19, 2021·Nature Communications·Fabian BlombachFinn Werner

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