Identification of a conserved DNA sulfur recognition domain by characterizing the phosphorothioate-specific endonuclease SprMcrA from Streptomyces pristinaespiralis

Molecular Microbiology
Hao YuXinyi He

Abstract

Streptomyces species have been valuable models for understanding the phenomenon of DNA phosphorothioation in which sulfur replaces a non-bridging oxygen in the phosphate backbone of DNA. We previously reported that the restriction endonuclease ScoMcrA from Streptomyces coelicolor cleaves phosphorothioate DNA and Dcm-methylated DNA at sites 16-28 nucleotides away from the modification sites. However, cleavage of modified DNA by ScoMcrA is always incomplete and accompanied by severe promiscuous activity on unmodified DNA. These features complicate the studies of recognition and cleavage of phosphorothioate DNA. For these reasons, we here characterized SprMcrA from Streptomyces pristinaespiralis, a much smaller homolog of ScoMcrA with a rare HRH motif, a variant of the HNH motif that forms the catalytic center of these endonucleases. The sulfur-binding domain of SprMcrA and its phosphorothioation recognition site were determined. Compared to ScoMcrA, SprMcrA has higher specificity in discerning phosphorothioate DNA from unmodified DNA, and this enzyme generally cuts both strands at a distance of 11-14 nucleotides from the 5' side of the recognition site. The HRH/HNH motif has its own sequence specificity in DNA hydrolysis, leading...Continue Reading

References

May 20, 1992·Journal of Molecular Biology·E SutherlandE A Raleigh
Jul 3, 1997·Nature·D A WahA K Aggarwal
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·D A WahA K Aggarwal
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BitinaiteI Schildkraut
Sep 10, 1999·The Journal of Biological Chemistry·A J PommerC Kleanthous
Dec 3, 1999·Nature Structural Biology·E A GalburtB L Stoddard
Dec 6, 2001·Journal of Molecular Biology·A J PommerC Kleanthous
Jan 10, 2002·Genes & Development·Adrian Bird
Aug 7, 2002·Biochemistry·Anthony H KeebleColin Kleanthous
Aug 12, 2004·Nucleic Acids Research·Lei ZhengJean-Louis Reymond
Aug 18, 2004·Journal of Molecular Biology·Betty W ShenBarry L Stoddard
Aug 17, 2005·Molecular Microbiology·Xiufen ZhouZixin Deng
Mar 21, 2007·Journal of Molecular Biology·Hsinchin Huang, Hanna S Yuan
May 23, 2007·Biochemistry·Jennifer H EastbergBarry L Stoddard
Sep 6, 2007·The Journal of Biological Chemistry·Matheshwaran SaravananValakunja Nagaraja
Oct 16, 2007·Nature Chemical Biology·Lianrong WangPeter C Dedon
Aug 30, 2008·Annual Review of Microbiology·Piet Borst, Robert Sabatini
Sep 6, 2008·Nature·Hideharu HashimotoXiaodong Cheng
Dec 17, 2008·Epigenetics : Official Journal of the DNA Methylation Society·Hideharu HashimotoXiaodong Cheng
Feb 9, 2010·PloS One·Siu-Hong ChanShuang-Yong Xu
Feb 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lianrong WangPeter C Dedon
Mar 8, 2011·Nucleic Acids Research·Aleksandra SzwagierczakFabio Spada
Apr 22, 2011·Progress in Molecular Biology and Translational Science·Lakshminarayan M IyerL Aravind
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Devora Cohen-KarniYu Zheng
Jul 10, 2012·Nucleic Acids Research·Xinqiang XieZhijun Wang
Nov 6, 2012·Nucleic Acids Research·Shuang-yong Xu, Yogesh K Gupta
Aug 22, 2013·Nucleic Acids Research·Kommireddy VasuValakunja Nagaraja
Aug 31, 2013·Nucleic Acids Research·Wil A M Loenen, Elisabeth A Raleigh
Nov 8, 2014·Nucleic Acids Research·Richard J RobertsDana Macelis

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