Identification of a copper-binding metallothionein in pathogenic mycobacteria.

Nature Chemical Biology
Ben GoldCarl Nathan

Abstract

A screen of a genomic library from Mycobacterium tuberculosis (Mtb) identified a small, unannotated open reading frame (MT0196) that encodes a 4.9-kDa, cysteine-rich protein. Despite extensive nucleotide divergence, the amino acid sequence is highly conserved among mycobacteria that are pathogenic in vertebrate hosts. We synthesized the protein and found that it preferentially binds up to six Cu(I) ions in a solvent-shielded core. Copper, cadmium and compounds that generate nitric oxide or superoxide induced the gene's expression in Mtb up to 1,000-fold above normal expression. The native protein bound copper within Mtb and partially protected Mtb from copper toxicity. We propose that the product of the MT0196 gene be named mycobacterial metallothionein (MymT). To our knowledge, MymT is the first metallothionein of a Gram-positive bacterium with a demonstrated function.

References

Jan 1, 1987·Experientia. Supplementum·M BeltraminiK Lerch
Jun 3, 1988·Science·J A Imlay, S Linn
May 10, 1985·Science·D H HamerJ E Lemontt
May 18, 1981·FEBS Letters·M Beltramini, K Lerch
Feb 1, 1995·Journal of Industrial Microbiology·J S Turner, N J Robinson
Jan 6, 1995·The Journal of Biological Chemistry·R Hassett, D J Kosman
Sep 1, 1994·Environmental Health Perspectives·C D KlaassenW C Kershaw
Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·J D MacMickingC F Nathan
Jan 7, 1998·The Journal of Experimental Medicine·S EhrtL W Riley
Aug 15, 1998·Biochemical and Biophysical Research Communications·C JacobB L Vallee
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E Graham, J E Clark-Curtiss
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·A T MilesV Rodilla
Jun 16, 2001·Advances in Microbial Physiology·N J RobinsonJ S Cavet
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·C A BlindauerP J Sadler
Jul 20, 2002·FEMS Microbiology Letters·Marie R B BinetRobert K Poole
Sep 5, 2002·Molecular Microbiology·Claudia A BlindauerNigel J Robinson
Mar 8, 2003·Molecular Microbiology·G Marcela Rodriguez, Issar Smith
Jun 28, 2003·FEMS Microbiology Reviews·Christopher Rensing, Gregor Grass
Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey M SchapiroFerric C Fang
Sep 4, 2003·The Journal of Experimental Medicine·Dirk SchnappingerGary K Schoolnik
Oct 25, 2003·Science·John D MacMickingJohn D McKinney
Jul 16, 2004·Journal of Proteome Research·Jonathan W Arthur, Marc R Wilkins
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
Dec 5, 2006·Nature Chemical Biology·Tong LiuDavid P Giedroc
Jan 5, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Claudia A BlindauerPeter J Sadler
Feb 12, 2008·Journal of Bacteriology·Sarah K WardAdel M Talaat
Mar 11, 2008·Cell Host & Microbe·Ruslana BrykCarl Nathan

❮ Previous
Next ❯

Citations

Oct 1, 2009·Chemical Reviews·Zhen MaDavid P Giedroc
Feb 17, 2009·Nature Chemical Biology
Sep 19, 2008·Nature Chemical Biology·Nigel J Robinson
Jun 18, 2011·Chemical Communications : Chem Comm·Svatava VoltrovaJiri Srogl
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Frank WolschendorfMichael Niederweis
Nov 6, 2009·The Journal of Biological Chemistry·Yan Zhang, Vadim N Gladyshev
Jun 19, 2013·Journal of Bacteriology·Jennifer L Rowland, Michael Niederweis
Jun 21, 2012·BMC Genomics·Kenneth D DoigTimothy P Stinear
Jan 26, 2013·PLoS Computational Biology·W Lee PangNitin S Baliga
Sep 2, 2011·Future Medicinal Chemistry·Tathagata Mukherjee, Helena Boshoff
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ben GoldCarl F Nathan
Feb 22, 2014·World Journal of Microbiology & Biotechnology·José Sebastián Dávila Costa, María Julia Amoroso
Feb 22, 2012·Cell Host & Microbe·Marie I SamanovicK Heran Darwin
Jan 29, 2013·Nature Chemical Biology·Yue FuDavid P Giedroc
Aug 21, 2013·Nature Chemical Biology·Shengmin ZhouNaoki Takaya
Mar 14, 2013·The Journal of Biological Chemistry·Teresita Padilla-BenavidesJosé M Argüello
Sep 10, 2013·Journal of Bacteriology·Alexander SpeerFrank Wolschendorf
May 20, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Claudia A Blindauer
Jun 3, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Mercè Capdevila, Sílvia Atrian
Feb 7, 2012·Trends in Microbiology·Hélène BotellaOlivier Neyrolles
Mar 1, 2012·Tuberculosis·Jennifer L Rowland, Michael Niederweis
Jul 19, 2011·Ecotoxicology and Environmental Safety·José Sebastián Dávila CostaCarlos Mauricio Abate
Nov 4, 2015·Trends in Microbiology·Marie I Samanovic, K Heran Darwin
Jul 14, 2010·Molecular Microbiology·Sarah K WardAdel M Talaat
Dec 21, 2010·Molecular Microbiology·Richard A FestaK Heran Darwin
Oct 16, 2012·Molecular Microbiology·Peng WangXin Li
Nov 16, 2010·Journal of Basic Microbiology·Andre SchmidtErika Kothe
Jul 23, 2011·Journal of Basic Microbiology·Andre SchmidtErika Kothe
Feb 24, 2015·Immunological Reviews·Olivier NeyrollesMichael Niederweis
Feb 24, 2015·Immunological Reviews·Christina E BaerChristopher M Sassetti
Aug 27, 2015·Nature·Nicolas VitaChristopher Dennison
Jan 28, 2014·Current Opinion in Chemical Biology·Joseph J Braymer, David P Giedroc
Oct 2, 2012·FEMS Microbiology Reviews·Helen Fones, Gail M Preston
Jul 31, 2009·Communicative & Integrative Biology·Ujjini ManjunathaClifton E Barry
Oct 29, 2010·Tuberculosis·Jocelyne M LewStewart T Cole
Nov 10, 2009·FEMS Microbiology Letters·Eun-Hae KimChristopher Rensing
Oct 22, 2015·Annual Review of Microbiology·Jordan B Jastrab, K Heran Darwin
Dec 20, 2016·Scientific Reports·Nicolas VitaChristopher Dennison

❮ Previous
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