PMID: 8601598Mar 1, 1996Paper

Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I

The Journal of Cell Biology
M N OdaD J Klionsky

Abstract

Aminopeptidase I (API) is a soluble leucine aminopeptidase resident in the yeast vacuole (Frey, J., and K.H. Rohm. 1978. Biochim. Biophys. Acta. 527:31-41). The precursor form of API contains an amino-terminal 45-amino acid propeptide, which is removed by proteinase B (PrB) upon entry into the vacuole. The propeptide of API lacks a consensus signal sequence and it has been demonstrated that vacuolar localization of API is independent of the secretory pathway (Klionsky, D.J., R. Cueva, and D.S. Yaver. 1992. J. Cell Biol. 119:287-299). The predicted secondary structure for the API propeptide is composed of an amphipathic alpha-helix followed by a beta-turn and another alpha-helix, forming a helix-turn-helix structure. With the use of mutational analysis, we determined that the API propeptide is essential for proper transport into the vacuole. Deletion of the entire propeptide from the API molecule resulted in accumulation of a mature-sized protein in the cytosol. A more detailed examination using random mutagenesis and a series of smaller deletions throughout the propeptide revealed that API localization is severely affected by alterations within the predicted first alpha-helix. In vitro studies indicate that mutations in this pr...Continue Reading

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Citations

Feb 13, 2001·Microscopy Research and Technique·J Vorísek
Jun 21, 2008·Parasitology Research·Marcel Marín-VillaYara M Traub-Cseko
Aug 28, 1998·Current Opinion in Cell Biology·S V Scott, D J Klionsky
Aug 28, 1998·Current Opinion in Cell Biology·M A De Matteis, J S Morrow
Jun 19, 2012·Cell Death and Differentiation·Kuninori Suzuki
Oct 3, 2007·Nature Cell Biology·Zhiping Xie, Daniel J Klionsky
Oct 26, 2005·Cell Death and Differentiation·T Yorimitsu, D J Klionsky
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·S V ScottD J Klionsky
Nov 30, 2011·The Journal of Biological Chemistry·Mariana Morales QuinonesPer E Stromhaug
Jan 22, 2005·Molecular Biology of the Cell·Tomohiro Yorimitsu, Daniel J Klionsky
Mar 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Wakana AdachiFuyuhiko Inagaki
Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D J Klionsky, Y Ohsumi
Apr 7, 2012·International Journal of Cell Biology·Midori Umekawa, Daniel J Klionsky
Dec 25, 2013·Cell Research·Yuchen FengDaniel J Klionsky
Jul 14, 1997·The Journal of Cell Biology·S V ScottD J Klionsky
Apr 20, 2001·The Journal of Cell Biology·J KimD J Klionsky
Feb 12, 2010·FEBS Letters·Melinda A Lynch-Day, Daniel J Klionsky
Aug 17, 2010·European Journal of Cell Biology·Iga PiekarskaBozenna Rempola
Dec 9, 2008·Biochemical and Biophysical Research Communications·Takuya KageyamaYoshinori Ohsumi
Jul 27, 2007·The FEBS Journal·Jean-Emmanuel SarryPhilip A Rea
Jul 26, 2015·Autophagy·Ming-Yuan SuChung-I Chang
Aug 31, 2000·Annual Review of Biochemistry·J Kim, D J Klionsky
Dec 9, 2014·Methods : a Companion to Methods in Enzymology·Rodrigo Soares GuimaraesFulvio Reggiori
Dec 14, 2002·Developmental Cell·Kuninori SuzukiYoshinori Ohsumi
Dec 14, 2002·Developmental Cell·Takahiro ShintaniDaniel J Klionsky
Jun 30, 2001·Molecular Cell·S V ScottD J Klionsky
Jul 16, 2013·Toxicology·Toshihiko AkiKoichi Uemura
Apr 7, 2009·Biochimica Et Biophysica Acta·Eduardo Cebollero, Fulvio Reggiori
Feb 3, 2009·Methods in Enzymology·Heesun Cheong, Daniel J Klionsky
Jun 6, 1998·The Journal of Biological Chemistry·D J Klionsky
Jul 9, 2017·Biotechnology and Bioengineering·Amy M EhrenworthPamela Peralta-Yahya
Jan 21, 2001·The Journal of Biological Chemistry·C Andrei-SelmerP V Schu

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