Sep 29, 2011

Identification of a DNA methylome signature of esophageal squamous cell carcinoma and potential epigenetic biomarkers

Epigenetics : Official Journal of the DNA Methylation Society
Sheila C LimaZdenko Herceg

Abstract

Esophageal squamous cell carcinoma (ESCC) is believed to arise from esophageal mucosa through accumulation of both genetic and epigenetic changes. DNA methylation is a critical epigenetic mechanism involved in key cellular processes and its deregulation has been linked to many human cancers, including ESCC. The aim of this study is to examine the global deregulation of methylation states in ESCC and identify potential early biomarkers. With this purpose, we performed a bead array analysis of more than 800 cancer-related genes in ten ESCC samples, ten matched surrounding tissues and four esophageal mucosa from healthy individuals. Pyrosequencing was used for validation of DNA methylation changes in up to 106 cases and 27 controls. A total of 37 CpG sites were found to be differentially methylated between tumors and surrounding tissues. These CpG sites were significantly enriched in genes related to several pathways including IL-10 anti-inflammatory signaling pathway and cell communication pathway. In addition, by comparing with healthy esophageal mucosa, we identified TFF1 gene as a potential early marker of ESCC. This is the first study to address methylation changes in ESCC in a large set of genes. Methylome analysis is shown ...Continue Reading

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Mentioned in this Paper

Biochemical Pathway
Protein Methylation
Neoplasms
DNA Methylation
Genetic Markers
Study of Epigenetics
Sequence Determinations, DNA
Squamous Cell Carcinoma
Malignant Neoplasms
Epigenesis, Genetic

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