Identification of a DNA segment containing the human DNA polymerase alpha gene

Biochemical and Biophysical Research Communications
H MiyazawaM Sekiguchi

Abstract

We isolated a temperature-sensitive mutant from mouse FM3A cells, designated as tsFT20, the DNA polymerase alpha activity of which is heat-labile. A hybrid clone (M6-39 cells) between human cells and tsFT20 cells contained one or two human chromosomes. M6-39 cells (primary hybrid) were exposed to gamma-ray and re-fused with tsFT20, after which we isolated two temperature-resistant secondary hybrids, both of which retained an identical minute portion of the human chromosome, 400-500 kilobase pairs (kbp). Immunological studies demonstrated that this secondary hybrid expressed human DNA polymerase alpha. Thus, the human DNA polymerase alpha gene was located within a DNA region of 400-500 kbp.

References

Dec 1, 1979·Archives of Biochemistry and Biophysics·A Weissbach
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·D KornT S Wang
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T S WangD Korn
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y MurakamiM Yamada
Jan 1, 1980·Annual Review of Biochemistry·M L DePamphilis, P M Wassarman
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K ShimizuM Wigler
Nov 16, 1984·Science·P K Liu, L A Loeb
Jul 18, 1984·Biochemical and Biophysical Research Communications·S Detera-WadleighS H Wilson

❮ Previous
Next ❯

Citations

Oct 5, 2013·Revue de synthèse·Osamu Kanamori
Mar 5, 1988·Journal of Molecular Biology·H TakeshimaY Matsukado
Sep 4, 2009·The Journal of Biological Chemistry·Christian S EichingerFumio Hanaoka
Sep 20, 2011·Expert Opinion on Investigational Drugs·Yoshiyuki MizushinaHiromi Yoshida
Aug 8, 2012·DNA Repair·Mutsuo Sekiguchi
Feb 1, 1990·Experimental Cell Research·P L HärkönenP D Darbre

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.