Identification of a DNA sequence motif required for expression of iron-regulated genes in pseudomonads

Molecular & General Genetics : MGG
I T RombelIain L Lamont

Abstract

Many bacteria respond to a lack of iron in the environment by synthesizing siderophores, which act as iron-scavenging compounds. Fluorescent pseudomonads synthesize strain-specific but chemically related siderophores called pyoverdines or pseudobactins. We have investigated the mechanisms by which iron controls expression of genes involved in pyoverdine metabolism in Pseudomonas aeruginosa. Transcription of these genes is repressed by the presence of iron in the growth medium. Three promoters from these genes were cloned and the activities of the promoters were dependent on the amounts of iron in the growth media. Two of the promoters were sequenced and the transcriptional start site were identified by S1 nuclease analysis. Sequences similar to the consensus binding site for the Fur repressor protein, which controls expression of iron-repressible genes in several gram-negative species, were not present in the promoters, suggesting that they are unlikely to have a high affinity for Fur. However, comparison of the promoter sequences with those of iron-regulated genes from other Pseudomonas species and also the iron-regulated exotoxin gene of P. aeruginosa allowed identification of a shared sequence element, with the consensus seq...Continue Reading

References

Mar 10, 2001·Environmental Microbiology·S RossbachM A Fisher
Jul 14, 2009·Journal of Bacteriology·Matt Shirley, Iain L Lamont
May 25, 2002·Journal of Bacteriology·Jiangsheng ShenK Poole
Jun 30, 2000·Biochemical and Biophysical Research Communications·M J Wilson, Iain L Lamont
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M WhiteleyE P Greenberg
Feb 2, 2002·Systematic and Applied Microbiology·G DevescoviV Venturi
Jun 14, 2014·FEMS Microbiology Reviews·María A LlamasIain L Lamont
Apr 28, 2007·Microbiology·Liang YangTim Tolker-Nielsen
Nov 17, 1999·Molecular Microbiology·M L Vasil, U A Ochsner
Jan 15, 1996·Molecular & General Genetics : MGG·R SextonF O'Gara
Nov 10, 1995·The Journal of Biological Chemistry·J B Neilands

Citations

Oct 1, 1979·Journal of Bacteriology·A A Mercer, J S Loutit
Jan 1, 1992·Journal of General Microbiology·I T Rombel, Iain L Lamont
Jan 1, 1991·Journal of Bacteriology·G J MurakawaD P Nierlich
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B GoldbergS B Calderwood
Mar 1, 1990·Molecular Microbiology·Douglas G StoreyB H Iglewski
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P C SalinasJ H Crosa
Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski
Aug 25, 1987·Nucleic Acids Research·D Grossberger
Nov 1, 1988·Infection and Immunity·K Poole, V Braun
Nov 1, 1988·Computer Applications in the Biosciences : CABIOS·R Smith
Oct 1, 1987·Journal of Bacteriology·S B Calderwood, J J Mekalanos
Dec 1, 1985·Computer Applications in the Biosciences : CABIOS·P A Stockwell
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A von GabainS N Cohen
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G L GrayH L Heyneker
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Jan 11, 1982·Nucleic Acids Research·R Staden, A D McLachlan
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg

Related Concepts

Gene Expression Regulation, Bacterial
Pseudomonas aeruginosa (antigen)
Transcription Repressor/Corepressor
Transcription, Genetic
Selfish DNA
Oligopeptides
Promoter
PAU07359
Pigments, Biological
Ligand Binding Domain

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.