Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding.

The Journal of Biological Chemistry
T S JuanH S Lichenstein

Abstract

CD14 is a 55-kDa glycoprotein that binds lipopolysaccharide (LPS) and enables LPS-dependent responses in a variety of cells. Monoclonal antibodies of CD14 such as 3C10 and MEM-18 are known to neutralize biological activity of CD14. Recently, it has been demonstrated that MEM-18 recognizes the LPS-binding site of CD14, between amino acids 57 and 64. It has also been shown that 3C10 recognizes a distinct epitope from that of MEM-18, indicating that 3C10 may yet define another functional domain of CD14. In order to identify the epitope for 3C10, we constructed a series of alanine substitution mutants of soluble CD14 (sCD14). BIAcore analyses showed that regions between amino acids 7 and 10 and between amino acids 11 and 14 are required for 3C10 binding. To assess the effect of altering the 3C10 epitope in CD14, we generated a stable cell line expressing a mutant sCD14 containing alanine substitutions in the region between amino acids 7 and 10, sCD14(7-10)A, and purified this protein to homogeneity. sCD14(7-10)A has impaired ability to mediate LPS-dependent IL-6 up-regulation in U373 cells, integrin activation in neutrophils, and NF-kappa B activation in U373 cells. Purified sCD14(7-10)A was, however, capable of forming a stable co...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O BagasraR J Pomerantz
Sep 1, 1986·The Journal of Experimental Medicine·P S TobiasR J Ulevitch
Dec 1, 1986·European Journal of Immunology·V BazilI Hilgert
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Jan 6, 1995·The Journal of Biological Chemistry·S Viriyakosol, T N Kirkland
Jan 20, 1995·The Journal of Biological Chemistry·T S JuanH S Lichenstein
Mar 10, 1995·The Journal of Biological Chemistry·T S JuanH S Lichenstein
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PuginP S Tobias
Jun 3, 1994·Journal of Immunological Methods·K P van KesselS D Wright
Jan 1, 1993·International Review of Cytology·M GrilliM J Lenardo

❮ Previous
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Citations

Nov 26, 2003·International Immunopharmacology·F A Fitzpatrick, Richard Wheeler
Jan 1, 1997·Journal of Periodontal Research·P S TobiasR J Ulevitch
Jul 15, 2003·Clinical Microbiology Reviews·Edwin S Van AmersfoortJohan Kuiper
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·A I TukhvatulinA L Gintsburg
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·D FilippM Julius
Aug 4, 2010·Cellular and Molecular Life Sciences : CMLS·Renato OstuniFrancesca Granucci
Apr 15, 2010·Annals of the New York Academy of Sciences·Jan StagstedHelle R Juul-Madsen
Nov 1, 2005·Biochemical and Biophysical Research Communications·Marcelo GonzalezEvelyne Bachère
Oct 18, 2005·Journal of Neuroimmunology·Clive BateAlun Williams
Aug 8, 1997·Journal of Toxicology and Environmental Health·P R Mayeux
Aug 15, 2008·Immunity·Mi Sun Jin, Jie-Oh Lee
Sep 10, 2013·Veterinary Immunology and Immunopathology·Adriana SilvaMartin O Furr
Apr 24, 2007·The International Journal of Biochemistry & Cell Biology·Izabela M NitaSabina M Janciauskiene
Jul 26, 2011·Free Radical Biology & Medicine·Paul K WittingSaul Benedict Freedman
Aug 25, 2001·Journal of Endotoxin Research·B Beutler, A Poltorak
May 16, 1998·The Journal of Biological Chemistry·R DziarskiP S Tobias
Feb 17, 1998·European Journal of Immunology·C BlondinN Haeffner-Cavaillon
Aug 28, 2002·The Journal of Biological Chemistry·Masashi MuroiKen-Ichi Tanamoto
Dec 7, 2007·The Journal of Biological Chemistry·Jianmin MengC James McKnight
Jun 1, 2001·The Journal of Biological Chemistry·C J ThomasA Surolia
Feb 1, 2000·The Journal of Biological Chemistry·S ViriyakosolT N Kirkland
Aug 2, 2002·The Journal of Biological Chemistry·Margreet A WolfertJames N Moore
Dec 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stacy L KelleyRichard I Tapping
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M Soler-RodriguezG R Klimpel
Mar 9, 2018·Frontiers in Immunology·Andrea N LoesMichael J Harms
Apr 5, 2019·Oxidative Medicine and Cellular Longevity·Dmitry S KabanovIsabella R Prokhorenko
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D CunninghamR P Darveau
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R I TappingP S Tobias
Jan 13, 2005·The Journal of Biological Chemistry·Jung-In KimJie-Oh Lee
Jan 22, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea N LoesMichael J Harms
Apr 1, 2008·The Journal of Experimental Biology·Kerry A DalyPeter Williamson

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