Identification of a Drosophila melanogaster ICE/CED-3-related protease, drICE

The EMBO Journal
A G Fraser, G I Evan

Abstract

Cysteine proteases of the ICE/CED-3 family (caspases) are required for the execution of programmed cell death (PCD) in a wide range of multicellular organisms. Caspases are implicated in the execution of apoptosis in Drosophila melanogaster by the observation that expression of baculovirus p35, a caspase inhibitor, blocks cell death in vivo in Drosophila. We report here the identification and characterization of drICE, a D. melanogaster caspase. We show that overexpression of drICE sensitizes Drosophila cells to apoptotic stimuli and that expression of an N-terminally truncated form of drICE rapidly induces apoptosis in Drosophila cells. Induction of apoptosis by rpr overexpression or by cycloheximide or etoposide treatment of Drosophila cells results in proteolytic processing of drICE. We further show that drICE is a cysteine protease that cleaves baculovirus p35 and Drosophila lamin DmO in vitro and that drICE is expressed at all the stages of Drosophila development at which PCD can be induced. Taken together, these results strongly argue that drICE is an apoptotic caspase that acts downstream of rpr. drICE is therefore the first unequivocal link between the molecular machinery of Drosophila cell death and the conserved machi...Continue Reading

References

Apr 3, 1992·Science·D P CerrettiL A Cannizzaro
Apr 3, 1992·Cell·G I EvanD C Hancock
Sep 20, 1988·Journal of Molecular Biology·N H Brown, F C Kafatos
Apr 29, 1994·Science·K WhiteH Steller
Feb 9, 1996·Science·K WhiteH Steller
Jul 1, 1996·Nature Structural Biology·J RotondaJ W Becker
Jul 15, 1996·Genes & Development·P ChenJ M Abrams
Feb 1, 1996·Current Opinion in Genetics & Development·A Takahashi, W C Earnshaw
Feb 1, 1996·Current Opinion in Neurobiology·A FraserG I Evan

❮ Previous
Next ❯

Citations

Feb 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Dawn M CooperCarl Lowenberger
Jun 2, 2007·Molecular Biology Reports·Dantong YangXiaofan Zhao
Jul 30, 2003·Developmental Biology·J S PetersonK McCall
Jun 20, 2002·Journal of Immunological Methods·Helena Richardson, Sharad Kumar
Sep 22, 1998·Current Biology : CB·R A OlieP Golstein
Oct 14, 2000·Cell Research·C Y Lee, E H Baehrecke
Jul 4, 1998·Scandinavian Journal of Immunology·V Depraetere, P Golstein
Jul 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Arjun GuhaThomas B Kornberg
Feb 20, 2009·The Journal of Biological Chemistry·Bilal AmarnehRobert B Rawson
Feb 17, 2000·The EMBO Journal·P MeierG I Evan
Sep 12, 2006·Development, Growth & Differentiation·Vicky E MpakouIssidora S Papassideri
Oct 14, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sharad Kumar
Sep 19, 2006·Molecular and Cellular Biology·Shu KondoMasayuki Miura
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·H Y Chang, X Yang
Jul 18, 2006·Annual Review of Cell and Developmental Biology·Bruce A Hay, Ming Guo
Feb 24, 2009·BMC Developmental Biology·Thomas Kessler, H Arno J Müller
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·H KanukaM Miura
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marco Di FruscioStephane Richard
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·W N HainingH Steller
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·T IgakiM Miura
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C J HawkinsB A Hay
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L DorstynS Kumar
Mar 31, 2005·Seminars in Cell & Developmental Biology·Peter CashioAndreas Bergmann
Feb 22, 2012·The Journal of Cell Biology·Anat Florentin, Eli Arama
Mar 20, 2002·The Journal of Cell Biology·Loretta DorstynSharad Kumar
Jul 26, 2000·The Journal of Cell Biology·S Y VernooyB A Hay
Jul 5, 2005·Expert Opinion on Investigational Drugs·L Denner
Jul 8, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Indrajit ChowdhuryGanapathy K Bhat
Oct 2, 2007·Developmental and Comparative Immunology·Jiann-Horng LeuChu-Fang Lo
Jun 16, 2011·Protein Science : a Publication of the Protein Society·Witold A Witkowski, Jeanne A Hardy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.