Identification of a follistatin-related protein from the tick Haemaphysalis longicornis and its effect on tick oviposition

Gene
Jinlin ZhouKozo Fujisaki

Abstract

The identification of ovary-associated molecules will lead to a better understanding of the physiology of tick reproduction and vector-pathogen interactions. A gene encoding a follistatin-related protein (FRP) was obtained by random sequencing from the ovary cDNA library of the tick Haemaphysalis longicornis. The full-length cDNA is 1157 bp, including an intact ORF encoding an expected protein with 289 amino acids. Three distinct domains were present in the deduced amino acids, namely, the follistatin-like domain, KAZAL, and two calcium-binding motifs, EFh. The sequence shows homology with the follistatin-related protein (FRP), which was thought to play some roles in the negative regulation of cellular growth. RT-PCR showed that the gene was expressed throughout the developing stages and mainly in the ovary as well as in fat body, hemocytes, salivary glands, and midgut. This gene was expressed in GST-fused recombinant protein with an expected size. The mouse antiserum against the recombinant protein recognized a 56-kDa native protein in both tick ovary and hemolymph. The recombinant proteins were found to have binding activity for both activin A and bone morphogenetic protein-2 (BMP-2). Silencing of FRP by RNAi showed a decreas...Continue Reading

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Citations

Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yan GengWen Ning
Aug 10, 2011·PloS One·Marc SylvaMaurice J B van den Hoff
Sep 17, 2013·Memórias do Instituto Oswaldo Cruz·Leah Theresa Sigle, Marcelo Ramalho-Ortigão
Mar 31, 2009·Insect Biochemistry and Molecular Biology·Haiyan GongKozo Fujisaki
Dec 25, 2007·Veterinary Parasitology·Haiyan GongKozo Fujisaki
Jul 27, 2007·Journal of Insect Physiology·Haiyan GongKozo Fujisaki
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Dec 17, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Girish Neelakanta, Hameeda Sultana
Mar 30, 2018·Cellular and Molecular Life Sciences : CMLS·Andrea MattiottiMaurice J B van den Hoff
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Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Jindřich ChmelařMichail Kotsyfakis
Jun 18, 2021·Experimental & Applied Acarology·Mayinuer TuerdiJinlin Zhou

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