Identification of a gene (lpt-3) required for the addition of phosphoethanolamine to the lipopolysaccharide inner core of Neisseria meningitidis and its role in mediating susceptibility to bactericidal killing and opsonophagocytosis

Molecular Microbiology
Fiona G MackinnonE Richard Moxon

Abstract

We have identified a gene, lpt-3, that is required for the addition of phosphoethanolamine to the 3-position (PEtn-3) on the beta-chain heptose (HepII) of the inner core lipopolysaccharide (LPS) of Neisseria meningitidis (Nm). The presence of this PEtn-3 substituent is characteristic of the LPS of a majority ( approximately 70%) of hypervirulent Nm strains, irrespective of capsular serogroup, and is required for the binding of a previously described monoclonal antibody (mAb B5) to a surface-accessible epitope. All strains of Nm that have PEtn-3 possess the lpt-3 gene. In some lpt-3-containing strains, the 3-position on HepII is preferentially substituted by glucose instead of PEtn, the result of lgtG phase variation mediated by slippage of a homopolymeric tract of cytidines. Inactivation of lpt-3 resulted in loss of PEtn-3, lack of reactivity with mAb B5 and conferred relative resistance to bactericidal killing and opsonophagocytosis by mAb B5 in vitro. Thus, the identification of lpt-3 has facilitated rigorous genetic, structural and immunobiological definition of an immunodominant epitope that is a candidate immunogen for inclusion in an LPS-based vaccine to protect against invasive meningococcal disease.

References

Nov 1, 1972·The Journal of Infectious Diseases·F A WyleJ P Lowenthal
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M C MaidenB G Spratt
Jul 8, 1998·European Journal of Biochemistry·A D CoxT J Inzana
Aug 4, 1998·European Journal of Biochemistry·W W WakarchukJ C Richards
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·A BanerjeeD C Stein
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Dec 22, 1999·Revista Do Instituto De Medicina Tropical De São Paulo·I V NisidaE S Umezawa

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Citations

Aug 30, 2002·European Journal of Biochemistry·Andrew D CoxJames C Richards
Apr 16, 2003·European Journal of Biochemistry·Andrew D CoxJames C Richards
Apr 28, 2010·The Journal of Biological Chemistry·Noëlle MistrettaBachra Rokbi
Jul 5, 2012·The Journal of Biological Chemistry·Nichollas E ScottStuart J Cordwell
Nov 28, 2012·Infection and Immunity·Thomas W CullenM Stephen Trent
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Finn Terje HeggeMichael Koomey
Sep 3, 2010·Molecular Microbiology·Gerald L MurrayBen Adler
Sep 27, 2012·Biochimica Et Biophysica Acta·Luce Farine, Peter Bütikofer
Dec 20, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Peter M PowerD W Hood
Feb 1, 2005·FEMS Immunology and Medical Microbiology·Rita TamayoJohn S Gunn
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Stephen Trent
Jan 30, 2018·Frontiers in Microbiology·Raquel Conde-ÁlvarezMaite Iriarte
Aug 15, 2018·Molecular & Cellular Proteomics : MCP·Alba HykollariKatharina Paschinger
Sep 26, 2019·Annals of the New York Academy of Sciences·Anandhi Anandan, Alice Vrielink

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