PMID: 9658002Jul 11, 1998Paper

Identification of a gene product induced by hard-surface contact of Colletotrichum gloeosporioides conidia as a ubiquitin-conjugating enzyme by yeast complementation

Journal of Bacteriology
Z M Liu, P E Kolattukudy

Abstract

The germinating conidia of many phytopathogenic fungi on hosts must differentiate into an infection structure called the appressorium in order to penetrate their hosts. Chemical signals, such as the host's surface wax or fruit ripening hormone, ethylene, trigger germination and appressorium formation of the avocado pathogen Colletotrichum gloeosporioides only after the conidia are in contact with a hard surface. What role this contact plays is unknown. Here, we describe isolation of genes expressed during the early stage of hard-surface treatment by a differential-display method and report characterization of one of these cloned genes, chip1 (Colletotrichum hard-surface induced protein 1 gene), which encodes a ubiquitin-conjugating enzyme. RNA blots clearly showed that it is induced by hard-surface contact and that ethylene treatment enhanced this induction. The predicted open reading frame (ubc1Cg) would encode a 16.2-kDa ubiquitin-conjugating enzyme, which shows 82% identity to the Saccharomyces cerevisiae UBC4-UBC5 E2 enzyme, comprising a major part of total ubiquitin-conjugating activity in stressed yeast cells. UBC1Cg can complement the proteolysis deficiency of the S. cerevisiae ubc4 ubc5 mutant, indicating that ubiquitin...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
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Citations

Aug 12, 2000·Journal of Bacteriology·Y K KimP E Kolattukudy
Jan 19, 2010·Molecular Plant Pathology·Zafer Dallal BashiMohammed H Borhan
Jul 1, 2001·Molecular Plant Pathology·A O Latunde-Dada
Aug 10, 2001·Fungal Genetics and Biology : FG & B·S W ThomasR P Oliver
Aug 12, 1999·Fungal Genetics and Biology : FG & B·S E PerfectJ R Green
Jul 3, 2021·Pathogens·K W Thilini ChethanaKevin D Hyde
Aug 12, 2008·Fungal Genetics and Biology : FG & B·Nataliya KucheryavaMatthew D Templeton

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