PMID: 9423846Jan 10, 1998Paper

Identification of a genetic locus essential for serotype b-specific antigen synthesis in Actinobacillus actinomycetemcomitans

Infection and Immunity
Y YoshidaT Koga

Abstract

A large gene cluster associated with the biosynthesis of the serotype-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 (serotype b) was cloned and characterized. Western blot analysis showed that Escherichia coli DH5alpha, containing a plasmid carrying this cluster, produced a polysaccharide which reacted with a monoclonal antibody directed against the SPA of A. actinomycetemcomitans Y4. High-performance liquid chromatography analysis indicated that the polysaccharide produced by an E. coli transformant, as well as A. actinomycetemcomitans Y4 SPA, was composed of rhamnose and fucose. Furthermore, using various derivatives of the plasmid, we demonstrated that the cloned 13-kb BssHII-BspHI fragment was indispensable for SPA synthesis in E. coli DH5alpha. The 24,909-bp nucleotide sequence, which included this fragment and its flanking regions, was determined. In the sequenced area, 24 open reading frames (ORFs) with the same orientation were found. Most of these were located sequentially within a short distance of each other. Many of the deduced amino acid sequences were similar to the gene products of the polysaccharide synthetic genes of other bacteria. The average G+C content (37.7%) of all 24 OR...Continue Reading

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Citations

Dec 18, 2009·Clinical and Vaccine Immunology : CVI·Jieh-Juen YuBernard P Arulanandam
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Jul 15, 2000·Oral Microbiology and Immunology·Y ShibataT Koga
Oct 7, 2003·Annual Review of Microbiology·Brian HendersonMichael Wilson
Apr 11, 2006·Canadian Journal of Microbiology·Rodrigo Estêvão TeixeiraPaula Prazeres Magalhães
Sep 2, 2003·Microbiology·Qun LiPeter R Reeves
Nov 7, 1998·Biochimica Et Biophysica Acta·Y NakanoT Koga
Apr 28, 2001·Journal of Clinical Microbiology·N SuzukiT Koga
Nov 26, 2003·Biochemical and Biophysical Research Communications·Leonilde M MoreiraIsabel Sá-Correia

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