Identification of a goblet cell-specific enhancer element in the rat intestinal trefoil factor gene promoter bound by a goblet cell nuclear protein.

The Journal of Biological Chemistry
H OgataD K Podolsky

Abstract

Intestinal trefoil factor (ITF) is selectively expressed in goblet cells of the small and large intestinal mucosa. Detailed analysis of the rat ITF (RITF) promoter was undertaken by transient transfection and gel mobility shift assays (GMSAs) using the goblet cell-like LS174T colon cancer-derived cell line. Various lengths of wild-type or mutant constructs of the 5'-flanking region were linked to the pXP2 reporter gene luciferase. Expression of -118 RITF was significantly decreased compared with -154 RITF, and transfection with an 18-base pair construct (-141 to -124) resulted in more than 5-fold greater expression than transfection with the promoterless pXP2 gene construct alone. Using various synthetic oligonucleotide mutants, GMSAs revealed that only a 9-base pair sequence (CCCCTCCCC) in this element was required for specific binding, overlapping but distinct from a Sp1-like element. GMSA demonstrated that this element was specifically bound by nuclear proteins from intestinal cells with a goblet cell-like phenotype. These studies demonstrate that a 9-base pair element (goblet cell response element) between -154 and -118 in the RITF promoter gene is a cis-active element bound by a distinct nuclear transcription factor and is...Continue Reading

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SuemoriD K Podolsky
Jul 1, 1988·The Biochemical Journal·L Thim
Dec 23, 1987·Nucleic Acids Research·J L Millán
Feb 25, 1987·Nucleic Acids Research·J M JeltschP Chambon
Sep 5, 1995·Biochemical and Biophysical Research Communications·T SeibC Welter
May 5, 1995·Biochemical and Biophysical Research Communications·H MashimoM C Fishman
Mar 17, 1995·The Journal of Biological Chemistry·J L MerchantD R Abraczinskas
Jul 1, 1993·Trends in Biochemical Sciences·W Hoffmann, F Hauser

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Citations

Feb 24, 2000·Biochimica Et Biophysica Acta·E D Al-azzehP Gött
Sep 18, 1998·Biochimica Et Biophysica Acta·S RibierasM C Rio
May 14, 1999·Gut·W M WongN A Wright
Dec 22, 2006·Regulatory Peptides·Tadahito ShimadaHideyuki Hiraishi
Feb 13, 2008·Journal of Cellular and Molecular Medicine·Hack Sun ChoiLi-Na Wei
Jun 5, 2004·Peptides·Shahin EmamiChristian Gespach
Jun 27, 2007·The American Journal of Pathology·Chang TongWancai Yang
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Jul 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hack Sun ChoiHorace H Loh
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·D Iwakiri, D K Podolsky
Apr 14, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M G MartínE M Wright
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·P G FalkJ I Gordon

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