Identification of a hepatitis C virus-reactive T cell receptor that does not require CD8 for target cell recognition

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Glenda G CallenderMichael I Nishimura

Abstract

Hepatitis C virus (HCV) has been reported to elicit B and T cell immunity in infected patients. Despite the presence of antiviral immunity, many patients develop chronic infections leading to cirrhosis, hepatocellular carcinoma, and liver failure that can require transplantation. We have previously described the presence of HLA-A2-restricted, HCV NS3-reactive cytotoxic T lymphocytes (CTL) in the blood of HLA-A2- liver transplantation patients that received an HLA-A2+ liver allograft. These T cells are analogous to the "allospecific" T cells that have been described in hematopoietic stem cell transplantation patients. It has been speculated that allospecific T cells express high-affinity T cell receptors (TCRs). To determine if our HCV-reactive T cells expressed TCRs with relatively high affinity for antigen, we identified and cloned a TCR from an allospecific HLA-A2-restricted, HCV:NS3:1406-1415-reactive CD8+ T cell clone and expressed this HCV TCR in Jurkat cells. Tetramer binding to HCV TCR-transduced Jurkat cells required CD8 expression, whereas antigen recognition did not. In conclusion, based on the reactivity of the TCR-transduced Jurkat cells, we have identified a TCR that transfers anti-HCV reactivity to alternate effec...Continue Reading

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Citations

Oct 7, 2008·Cancer Immunology, Immunotherapy : CII·Tamson V MooreMichael I Nishimura
Jan 5, 2011·Cancer Immunology, Immunotherapy : CII·Andrew ZlozaJosé A Guevara-Patiño
Aug 21, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Anna PasettoMargaret Chen
Feb 5, 2016·Cancer Immunology, Immunotherapy : CII·Timothy T SpearMichael I Nishimura
Aug 6, 2013·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Gustaf AhlénMatti Sällberg
Feb 20, 2007·Molecular Aspects of Medicine·Boris Engels, Wolfgang Uckert
Oct 31, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Michael G Hughes, Hugo R Rosen
Sep 22, 2005·Journal of Translational Medicine·Mark D McKeeMichael I Nishimura
Jun 24, 2004·Journal of Translational Medicine·W Martin KastFrancesco M Marincola
May 4, 2016·Cancer Immunology, Immunotherapy : CII·Timothy T SpearMichael I Nishimura
May 25, 2005·Cancer Letters·Barbara L F KaplanMichael I Nishimura
Jun 22, 2017·Cancer Immunology, Immunotherapy : CII·Timothy T SpearMichael I Nishimura
Jun 3, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yuan WangBrian M Baker
Oct 2, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna PasettoMargaret Chen
Dec 20, 2019·Journal of Hematology & Oncology·Qinghua HeJinsheng Weng
Dec 6, 2006·Cancer Research·Gretchen E LyonsMichael I Nishimura

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