PMID: 4362642Mar 1, 1974Paper

Identification of a high affinity nuclear acceptor site for estrogen receptor of calf uterus

Proceedings of the National Academy of Sciences of the United States of America
G A PucaE Nola

Abstract

By means of affinity chromatography, specific nuclear acceptor sites for estradiol receptors are identified in a fraction that can be solubilized from purified nuclei with 2 M NaCl. Interaction between these acceptor sites and crude or partially purified estradiol receptor shows a high association constant (over 10(9) M). Receptor-acceptor interaction is dependent on physiological concentrations of 17beta-estradiol; it is disrupted by high ionic strength. The nuclear acceptor sites appear to be protein in nature and exist in 5- to 10-fold excess over the estrogen binding sites present in the cytosol. Single- or double-stranded DNA does not bind estrogen-receptor complexes. Acceptor sites appear to be associated with basic nuclear proteins as judged by hydroxyapatite chromatography. The nuclear acceptor sites probably represent less than 0.1% of the purified basic proteins from the nucleus.

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Citations

Jul 5, 1977·Molecular and Cellular Biochemistry·E H Charreau, A Baldi
Jul 1, 1979·Journal of Steroid Biochemistry·T C SpelsbergG Martin-Dani
Sep 1, 1981·Journal of Steroid Biochemistry·L D KlineY A Lefebvre
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Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K R YamamotoG M Tomkins
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J C Azizkhan, M Khagsbrun
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J KallosV P Hollander
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I M FeaversJ P Jost
Oct 20, 2010·The Journal of Biological Chemistry·Brian York, Bert W O'Malley
Nov 1, 1976·Steroids·F Gannon, J Gorski
Jan 1, 1981·Molecular and Cellular Endocrinology·R E Leake
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Feb 24, 1976·Biochemistry·J V Juliano, G M Stancel
Mar 1, 1980·The British Journal of Surgery·R A HawkinsA P Forrest

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