Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum

Microbial Cell Factories
Julen Tomás-CortázarHéctor Rodríguez

Abstract

Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. We have identified and expressed in E. coli a tannase from the oral microbiota member Fusobacterium nucleatum subs. polymorphum (TanBFnp). TanBFnp is the first tannase identified in an oral pathogen. Sequence analyses revealed that it is closely related to other bacterial tannases. The enzyme exhibits biochemical properties that make it an interesting target for industrial use. TanBFnp has one of the highest specific activities of all bacterial tannases described to date and shows optimal biochemical properties such as a high thermal stability: the enzyme keeps 100% of its activity after prolonged incubations at different temperatures up to 45 °C. TanBFnp also shows a wide temperature range of activity, maintaining above 80% of its maximum activity between 22 and 55 °C. The use of a panel of 27 esters of phenolic acids demonstrated activity of TanBFnp only against esters of gallic and protocatechuic acid, including tannic acid, gallocatechin gallate and epigallocatechin gallate. Overall, TanBFnp possesses biochemical properties...Continue Reading

References

Mar 1, 1988·Analytical Biochemistry·K H Inoue, A E Hagerman
Jan 1, 1997·Advances in Applied Microbiology·P K Lekha, B K Lonsane
Oct 6, 1998·Critical Reviews in Food Science and Nutrition·K T ChungY Lin
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jun 21, 2006·Systematic Biology·Maria Anisimova, Olivier Gascuel
Oct 13, 2006·BMC Bioinformatics·François ChevenetRichard Christen
Mar 16, 2007·Microbial Cell Factories·Pernilla TurnerEva Nordberg Karlsson
May 29, 2007·Applied Microbiology and Biotechnology·Cristóbal N AguilarJuan C Contreras-Esquivel
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
May 19, 2009·Diagnostic Microbiology and Infectious Disease·Norihisa NoguchiMasanori Sasatsu
Jul 16, 2009·Journal of Agricultural and Food Chemistry·José Antonio CurielRosario Muñoz
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
May 6, 2010·Accident; Analysis and Prevention·Rocío Sánchez-MangasAntonio Martín Arroyo
Jan 21, 2011·Journal of Experimental & Clinical Cancer Research : CR·Ahmed S AbdulamirFatimah Abu Bakar
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Apr 30, 2013·Journal of Dental Research·Y W Han, X Wang
May 8, 2013·Journal of Molecular Biology·Bin RenQianming Chen
Oct 12, 2013·Applied Microbiology and Biotechnology·Diego Javier JiménezJan Dirk van Elsas
Mar 13, 2014·Applied and Environmental Microbiology·Natalia JiménezRosario Muñoz
Nov 22, 2014·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Oct 23, 2016·British Journal of Pharmacology·Antonella SmeriglioDomenico Trombetta
May 17, 2017·Journal of Infection and Public Health·Norihisa NoguchiMasanori Sasatsu
Jun 24, 2017·Science·Nitzan KoppelEmily P Balskus
Sep 20, 2017·Scientific Reports·Hongsen ZhaoJingping Liang

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Citations

Nov 22, 2018·Applied Microbiology and Biotechnology·Blanca de Las RivasRosario Muñoz
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Sunny DhimanAbhishek Kumar Singh
Oct 8, 2018·Applied Microbiology and Biotechnology·Valeria PrigioneGiovanna Cristina Varese

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Methods Mentioned

BETA
gel filtration
PCR
protein assay

Software Mentioned

Gblocks
CDD
TreeDyn
PhyML
CLUSTAL omega
BLASTp
Phylogeny
WebLogo package
Python
CLUSTAL

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