Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1)

doi:10.1371/journal.pone.0021289

Jun 30, 2011

Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1)

PloS One

Lilian Lacerda BuenoErika Martins Braga

Get PDF

Abstract

Apical membrane antigen 1 (AMA-1) is considered to be a major candidate antigen for a malaria vaccine. Previous immunoepidemiological studies of naturally acquired immunity to Plasmodium vivax AMA-1 (PvAMA-1) have shown a higher prevalence of specific antibodies to domain II (DII) of AMA-1. In the present study, we confirmed that specific antibody responses from naturally infected individuals were highly reactive to both full-length AMA-1 and DII. Also, we demonstrated a strong association between AMA-1 and DII IgG and IgG subclass responses. We analyzed the primary sequence of PvAMA-1 for B cell linear epitopes co-occurring with intrinsically unstructured/disordered regions (IURs). The B cell epitope comprising the amino acid sequence 290-307 of PvAMA-1 (SASDQPTQYEEEMTDYQK), with the highest prediction scores, was identified in domain II and further selected for chemical synthesis and immunological testing. The antigenicity of the synthetic peptide was identified by serological analysis using sera from P. vivax-infected individuals who were knowingly reactive to the PvAMA-1 ectodomain only, domain II only, or reactive to both antigens. Although the synthetic peptide was recognized by all serum samples specific to domain II, se...Continue Reading

References66
Citations17

References

Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection
(opens in new tab)
Microbes and Infection
Bruno C MúfaloIrene S Soares
Nucleotide sequence polymorphism at the apical membrane antigen-1 locus reveals population history of Plasmodium vivax in Thailand
(opens in new tab)
Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Chaturong PutaporntipAustin L Hughes
A role for apical membrane antigen 1 during invasion of hepatocytes by Plasmodium falciparum sporozoites
(opens in new tab)
The Journal of Biological Chemistry
Olivier SilvieDominique Mazier
Plasmodium genomics: latest milestone
(opens in new tab)
Nature Reviews. Microbiology
A Pain, Christiane Hertz-Fowler
Immunization with parasite-derived apical membrane antigen 1 or passive immunization with a specific monoclonal antibody protects BALB/c mice against lethal Plasmodium yoelii yoelii YM blood-stage infection
(opens in new tab)
Infection and Immunity
D L NarumA A Holder

See more references from this paper

References66
Citations17

Citations

Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico
(opens in new tab)
Parasites & Vectors
Lilia González-CerónFrida Santillán
In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9
(opens in new tab)
PloS One
Rodrigo Nunes Rodrigues-da-SilvaJosué da Costa Lima-Junior
Designing and modeling of complex DNA vaccine based on tropomyosin protein of Boophilus genus tick
(opens in new tab)
Applied Biochemistry and Biotechnology
Mohamamd Mahdi RanjbarMaryam Taheri
High levels of IgG3 anti ICB2-5 in Plasmodium vivax-infected individuals who did not develop symptoms
(opens in new tab)
Malaria Journal
Fernanda G VersianiPaulo A Nogueira
Computational tools for modern vaccine development
(opens in new tab)
Human Vaccines & Immunotherapeutics
SunitaPratyoosh Shukla

See more citations of this paper

Mentioned in this Paper

Monoclonal Antibodies

Plasmodium

Monoclonal antibodies, antineoplastic

Merozoites

Glycerin

Carboxy-Terminal Amino Acid

Pellet Formation

Immunologic Adjuvants

Phyllostachys vivax

Inclusion Bodies

About this Paper

PMID: 21713006

DOI: 10.1371/journal.pone.0021289

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1)

Abstract

References

Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

Nucleotide sequence polymorphism at the apical membrane antigen-1 locus reveals population history of Plasmodium vivax in Thailand

A role for apical membrane antigen 1 during invasion of hepatocytes by Plasmodium falciparum sporozoites

Plasmodium genomics: latest milestone

Immunization with parasite-derived apical membrane antigen 1 or passive immunization with a specific monoclonal antibody protects BALB/c mice against lethal Plasmodium yoelii yoelii YM blood-stage infection

See more references from this paper

Citations

Diversity and evolutionary genetics of the three major Plasmodium vivax merozoite genes participating in reticulocyte invasion in southern Mexico

In silico Identification and Validation of a Linear and Naturally Immunogenic B-Cell Epitope of the Plasmodium vivax Malaria Vaccine Candidate Merozoite Surface Protein-9

Designing and modeling of complex DNA vaccine based on tropomyosin protein of Boophilus genus tick

High levels of IgG3 anti ICB2-5 in Plasmodium vivax-infected individuals who did not develop symptoms

Computational tools for modern vaccine development

See more citations of this paper

Mentioned in this Paper

About this Paper

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Antimalarial Agents

Aminoglycosides (ASM)

Babesiosis

Anti-Dfs70 Antibodies

Antimalarial Agents (ASM)

Aminoglycosides

Avian Influenza: Innate Immune Adjuvant (ASM)

Related Papers

A recombinant vaccine based on domain II of Plasmodium vivax Apical Membrane Antigen 1 induces high antibody titres in mice

Population genetic structure and polymorphism analysis of gene encoding apical membrane antigen-1 (AMA-1) of Iranian Plasmodium vivax wild isolates

Apical membrane antigen 1: a malaria vaccine candidate in review

In immunization with Plasmodium falciparum apical membrane antigen 1, the specificity of antibodies depends on the species immunized

Structural comparison of apical membrane antigen 1 orthologues and paralogues in apicomplexan parasites