PMID: 2335245Apr 24, 1990Paper

Identification of a highly reactive sulphydryl group in human placental glutathione transferase by a site-directed fluorescent reagent

FEBS Letters
M Lo BelloG Federici

Abstract

A fluorescent maleimide derivative, N-(4-anilino-1-naphthyl) maleimide (ANM), a specific probe for thiol groups, reacted with human placental glutathione transferase (GST, EC 2.5.1.18), causing a complete inactivation of the enzyme in a few minutes. The modified enzyme was denatured, alkylated and digested with (L-1-tosylamide-2-phenylethyl chloromethyl ketone)-trypsin. The tryptic digest was analysed by HPLC and a fluorescent peptide was obtained. The sequence of this peptide allowed us, by a comparison with a well known primary structure, to assign the position 47 to the most reactive cysteine of GST enzyme.

References

Oct 1, 1988·The Biochemical Journal·I G CowellJ B Taylor
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B MannervikH Jörnvall
Jan 1, 1981·Methods in Enzymology·W H Habig, W B Jakoby

❮ Previous
Next ❯

Citations

Jan 5, 1996·Chemico-biological Interactions·C J RompelbergP J van Bladeren
May 22, 1992·Biochimica Et Biophysica Acta·M Lo BelloG Federici
Mar 16, 1994·Biochimica Et Biophysica Acta·M C Wilce, M W Parker
Apr 21, 1999·Biochimica Et Biophysica Acta·T BucciarelliC Di Ilio
Jan 8, 2008·Journal of Molecular Biology·Usama M HegazyBengt Mannervik
Jun 29, 2012·Chembiochem : a European Journal of Chemical Biology·Indalecio Quesada-SorianoLuís García-Fuentes
Jun 12, 2010·Journal of Molecular Recognition : JMR·Indalecio Quesada-SorianoLuis García-Fuentes
Dec 16, 1991·Biochemical and Biophysical Research Communications·K H KongK Takahashi
Jul 12, 1996·Biochemical Pharmacology·C di IlioA Aceto
Oct 3, 2006·Chemico-biological Interactions·M E LetelierP Aracena-Parks
Dec 17, 2003·The Journal of Biological Chemistry·Usama M HegazyGun Stenberg
Feb 17, 1998·Chemico-biological Interactions·M L van IerselP J van Bladeren
Apr 11, 2003·Chemico-biological Interactions·Jelmer J van ZandenIvonne M C M Rietjens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.