PMID: 2335245Apr 24, 1990Paper

Identification of a highly reactive sulphydryl group in human placental glutathione transferase by a site-directed fluorescent reagent

FEBS Letters
M Lo BelloG Federici


A fluorescent maleimide derivative, N-(4-anilino-1-naphthyl) maleimide (ANM), a specific probe for thiol groups, reacted with human placental glutathione transferase (GST, EC, causing a complete inactivation of the enzyme in a few minutes. The modified enzyme was denatured, alkylated and digested with (L-1-tosylamide-2-phenylethyl chloromethyl ketone)-trypsin. The tryptic digest was analysed by HPLC and a fluorescent peptide was obtained. The sequence of this peptide allowed us, by a comparison with a well known primary structure, to assign the position 47 to the most reactive cysteine of GST enzyme.


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Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B MannervikH Jörnvall
Jan 1, 1981·Methods in Enzymology·W H Habig, W B Jakoby

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