Identification of a hormonally regulated luteinizing hormone/human chorionic gonadotropin receptor mRNA binding protein. Increased mrna binding during receptor down-regulation.

The Journal of Biological Chemistry
John C Kash, K M Menon

Abstract

To elucidate the molecular events associated with the regulation of luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor mRNA stability during hCG-induced receptor down-regulation, we have identified an LH/hCG receptor-specific mRNA binding protein. Proteins were isolated from control and down-regulated rat ovary and were incubated with in vitro transcribed RNAs corresponding to the full-length LH/hCG receptor, as well as 5'- and 3'-truncated receptor forms. Resultant ribonucleoprotein complexes were analyzed by RNA gel mobility shift. A prominent Mr 50,000 ribonucleoprotein complex was identified with the following characteristics: 1) specificity for LH/hCG receptor open reading frame sequences located between nucleotides 102 and 282; 2) lack of competition by nonspecific RNAs; 3) a 3-fold increase in RNA binding activity during hCG-induced receptor down-regulation; and 4) limited tissue expression. This report describes the first evidence of an LH/hCG receptor mRNA binding protein, which we term LRBP-1, for luteinizing hormone receptor RNA binding protein-1. This protein is a candidate for a trans-acting factor involved in the hormonal regulation of LH/hCG receptor mRNA stability in rat ovary.

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Citations

Mar 16, 2005·Molecular and Cellular Endocrinology·H PeegelK M J Menon
Feb 22, 2012·Molecular and Cellular Endocrinology·K M J Menon, Bindu Menon
Oct 24, 2006·Molecular and Cellular Endocrinology·K M J MenonHelle Peegel
Jun 24, 2010·Molecular and Cellular Endocrinology·K M J MenonM Harada
Jan 30, 2004·The Journal of Biological Chemistry·Anil K Nair, K M J Menon
Apr 12, 2002·Endocrine Reviews·Mario AscoliDeborah L Segaloff
Sep 26, 2013·Endocrinology·Bindu MenonK M J Menon
Oct 3, 1999·Physiological Reviews·A J Morris, C C Malbon
Jun 1, 2019·The Journal of Clinical Endocrinology and Metabolism·Yin-Xia LiK M J Menon

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