PMID: 6407986Jan 1, 1983Paper

Identification of a human Ia antigen that is different from HLA-DR and DC antigens

Immunogenetics
M W MakgobaA J McMichael

Abstract

A monoclonal antibody, MHM4, identified a cell surface antigen present on B cells and not resting T cells. It precipitated two polypeptide chains of 34 000 and 28 000 daltons from B lymphoblastoid cells. This antibody bound to all B-cell lines tested, except those homozygous for HLA-DR7. Saturation binding assays and Scatchard plots of MHM4 binding to cells that did not carry HLA-DR7 indicated that this antibody bound less than the total surface Ia antigen. When the antibody was competed with eight other HLA-D-specific antibodies, the epitope recognized was shown to be distinct. Two-dimensional gel analysis revealed that a simple pattern of spots was precipitated, unlike the complex patterns obtained with other HLA-D-specific antibodies. The alpha and beta spots were different from those precipitated by HLA-DR- or DC-specific antibodies. It is argued that the MHM4 antigen is the product of an HLA locus that is distinct from HLA-DR and DC. Its relationship with HLA-SB is discussed.

References

Dec 1, 1978·The Journal of Experimental Medicine·R TosiD Pressman
Jan 1, 1979·Immunological Reviews·F M BrodskyW F Bodmer
Oct 19, 1979·Science·J W UhrR G Cook
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D J Charron, H O McDevitt
Dec 1, 1977·Australian and New Zealand Journal of Medicine·P J MorrisI R Mackay
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E J Yunis, D B Amos
Feb 1, 1974·European Journal of Immunology·J C Jensenius, A F Williams
Jan 1, 1980·The Journal of Experimental Medicine·D A Shackelford, J L Strominger
Feb 10, 1983·Nature·M W MakgobaP J Morris
Aug 1, 1981·Human Immunology·J M PesandoS F Schlossman
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A ShackelfordJ L Strominger

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Citations

Dec 1, 1989·Molecular Immunology·J E Hildreth, J A Hyman
Nov 1, 1993·AIDS Research and Human Retroviruses·R J Orentas, J E Hildreth
Sep 1, 1995·AIDS Research and Human Retroviruses·M M Guo, J E Hildreth
Dec 8, 1983·Nature·S Shaw, A McMichael
Jul 1, 1985·Immunological Reviews·J TrowsdaleJ Bodmer
Jan 1, 1989·Archives of Biochemistry and Biophysics·S M ManeJ T August

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