PMID: 9658096Jul 11, 1998Paper

Identification of a human immunodeficiency virus type 2 (HIV-2) encapsidation determinant and transduction of nondividing human cells by HIV-2-based lentivirus vectors

Journal of Virology
E M PoeschlaF Wong-Staal

Abstract

Although previous lentivirus vector systems have used human immunodeficiency virus type 1 (HIV-1), HIV-2 is less pathogenic in humans and is amenable to pathogenicity testing in a primate model. In this study, an HIV-2 molecular clone that is infectious but apathogenic in macaques was used to first define cis-acting regions that can be deleted to prevent HIV-2 genomic encapsidation and replication without inhibiting viral gene expression. Lentivirus encapsidation determinants are complex and incompletely defined; for HIV-2, some deletions between the major 5' splice donor and the gag open reading frame have been shown to minimally affect encapsidation and replication. We find that a larger deletion (61 to 75 nucleotides) abrogates encapsidation and replication but does not diminish mRNA expression. This deletion was incorporated into a replication-defective, envelope-pseudotyped, three-plasmid HIV-2 lentivirus vector system that supplies HIV-2 Gag/Pol and accessory proteins in trans from an HIV-2 packaging plasmid. The HIV-2 vectors efficiently transduced marker genes into human T and monocytoid cell lines and, in contrast to a murine leukemia virus-based vector, into growth-arrested HeLa cells and terminally differentiated hum...Continue Reading

References

Sep 1, 1990·Journal of Virology·G A VigliantiJ I Mullins
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ColombiniF Wong-Staal
Jun 1, 1994·Blood Reviews·T L Holyoake, M J Alcorn
Jan 1, 1995·Advances in Experimental Medicine and Biology·M StevensonM Emerman
Jan 1, 1994·Methods in Cell Biology·D G CookR W Doms
Feb 23, 1995·Nature·R HeimR Y Tsien
Apr 16, 1994·Lancet·P J KankiJ L Sankalé
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N K HeinzingerM Emerman
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R TalbottF Wong-Staal
Apr 1, 1993·AIDS Research and Human Retroviruses·P ChatterjeeS K Arya
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L NaldiniI M Verma
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P CorbeauF Wong-Staal
Nov 5, 1997·Nature Biotechnology·R ZuffereyD Trono

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Citations

Feb 26, 2000·Annual Review of Genetics·H TangF Wong-Staal
Mar 9, 2002·Journal of Virology·Melinda Butsch, Kathleen Boris-Lawrie
Feb 8, 2003·DNA and Cell Biology·Ricardo Quinonez, Richard E Sutton
May 22, 2004·Journal of Biomedical Science·Andrew M L LeverJing Zhao
Dec 23, 2006·Retrovirology·James B Whitney, Mark A Wainberg
Dec 19, 2001·Human Gene Therapy·N LoewenE M Poeschla
Dec 1, 2001·Expert Opinion on Biological Therapy·S PandyaV Planelles
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A Pfeifer, I M Verma
Oct 3, 2002·Annual Review of Pharmacology and Toxicology·Neeltje A Kootstra, Inder M Verma
Nov 5, 2002·Journal of Virology·Iris KemlerEric M Poeschla
Aug 3, 2005·Nature Reviews. Microbiology·Victoria D'Souza, Michael F Summers
Feb 17, 2007·Nature Reviews. Microbiology·Youichi Suzuki, Robert Craigie
Jan 5, 2006·Human Gene Therapy·Kevin V Morris, David J Looney
Aug 26, 2003·Journal of Medical Virology·Jenice D'CostaSuresh K Arya
Feb 12, 2008·Current Protocols in Molecular Biology·Ali Ramezani, Robert G Hawley
Aug 21, 2002·Journal of Virology·Manuel LlanoEric M Poeschla
Apr 18, 2012·The Biochemical Journal·Toshie SakumaYasuhiro Ikeda
Mar 12, 1999·Journal of Virology·S M WhiteV Planelles
Nov 23, 2020·Journal of Molecular Biology·Chijioke N UmunnakweWei-Shau Hu
Jan 28, 2021·Methods and Protocols·Ishani Dasgupta, Anushila Chatterjee
Jul 18, 2017·Human Gene Therapy Methods·Elena Herrera-CarrilloBen Berkhout
May 18, 2000·Reviews in Medical Virology·G PalùM Pizzato
May 15, 2021·Drug Delivery and Translational Research·Fahima Danesh PouyaMohadeseh Nemati

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