Identification of a key domain in annexin and 14-3-3 proteins that stimulate calcium-dependent exocytosis in permeabilized adrenal chromaffin cells

FEBS Letters
D RothR D Burgoyne

Abstract

Calcium-dependent secretion in digitonin-permeabilized adrenal chromaffin cells is stimulated by exogenous annexin II and 14-3-3 proteins. These proteins share a conserved domain that has been suggested to be involved in specific protein-protein interactions. We examined whether this domain was involved in secretion by using a synthetic peptide (P16) of sequence KGDYQKALLYLCGGDD corresponding to the C-terminus of annexin II. P16, but not truncated peptides, prevented the stimulation of secretion by 14-3-3 proteins and produced a partial inhibition of control secretion. These data suggest that the shared annexin/14-3-3 domain is important in the mechanisms controlling Ca(2+)-dependent secretion and may play a key role in protein-protein interactions during exocytosis.

References

Feb 18, 1992·Biochemistry·D D SchlaepferH T Haigler
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C LevequeN Martin-Moutot
Dec 1, 1992·Trends in Biochemical Sciences·A AitkenJ Soll
Jul 22, 1991·Biochimica Et Biophysica Acta·R D Burgoyne
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Mochly-RosenJ Lopez
Apr 12, 1990·Nature·A AitkenA Toker
Dec 1, 1990·Biochemical Society Transactions·R D Burgoyne, A Morgan
Jan 15, 1993·Cell·L A ElferinkR H Scheller

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Citations

Aug 1, 1995·Molecular and Cellular Biochemistry·D M Waisman
Jun 17, 1998·Molecular Neurobiology·E M Skoulakis, R L Davis
Dec 3, 2003·Trends in Parasitology·María del Mar Siles-Lucas, Bruno Gottstein
Jan 20, 2000·Trends in Cardiovascular Medicine·K A Hajjar, S Krishnan
Sep 30, 2000·Parasite Immunology·M Siles-LucasM Breijo
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·K NakanoM W Bitensky
Mar 9, 1994·Annals of the New York Academy of Sciences·R D BurgoyneD Roth
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Robert J. Ferl
Dec 25, 2002·Biological & Pharmaceutical Bulletin·Toshihiro Fujii, Takayuki Ueeda
May 20, 2014·Journal of Cell Communication and Signaling·Jessica K HitchcockGeorgia Schäfer
Jan 10, 2001·The Journal of Cell Biology·J GarinM Desjardins
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Dorota Konopka-PostupolskaAndreas Hofmann
Oct 22, 2016·Archives of Insect Biochemistry and Physiology·Abel Trujillo-OcampoFidel de la Cruz Hernández-Hernández
Apr 20, 2017·International Journal of Molecular Sciences·Dorota Konopka-Postupolska, Greg Clark
Mar 23, 2013·International Journal of Molecular Sciences·Alamelu BharadwajDavid Waisman
Jun 13, 1997·The Journal of Biological Chemistry·G KassamD M Waisman
Apr 1, 1996·Histochemistry and Cell Biology·M KnochelR D Burgoyne
Jun 1, 1994·DNA and Cell Biology·P H RoseboomD C Klein
Oct 29, 1998·Methods : a Companion to Methods in Enzymology·A TandonW E Balch

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