Abstract
Motility has been implicated in the invasive process of Borrelia burgdorferi (Bb), the etiologic agent of Lyme disease. To identify Bb motility related genes, we used a method termed 'semi-random PCR chromosome walking' (SRPCW) to walk through a large motility gene cluster. The major advantage of this approach over other PCR walking methods is that it employs a secondary PCR amplification of cloned fragments which can be readily sequenced and analyzed. Starting with a primer specific to flgE, we identified and sequenced 14 open reading frames (ORFs) spanning 11 kb downstream of the flgE gene. The genes identified include flbD, motA, motB, fliL, fliM, fliN, fliZ, fliP, fliQ, fliR, flhB, flhA, flhF and flbE. Twelve of the deduced proteins shared extensive homology with flagellar proteins from other bacteria. The gene products and order of genes within this cluster are most similar to those of Treponema pallidum (Tp) and Bacillus subtilis (Bs). One of the unique genes identified, flbD, demonstrated homology to an ORF from the same operon of Tp. Another ORF, flbE, showed similarity to genes from both Tp and Bs. RT-PCR and primer extension analysis revealed that this gene cluster is transcribed as a single unit indicating that it is...Continue Reading
References
Sep 1, 1992·Molecular Microbiology·P B CarpenterG W Ordal
Dec 20, 1992·Journal of Molecular Biology·A DingwallL Shapiro
Jan 1, 1992·Molecular Microbiology·D S Bischoff, G W Ordal
Jul 1, 1991·The Journal of Clinical Investigation·A SadzieneA G Barbour
Jun 11, 1991·Nucleic Acids Research·J D ParkerG C Burmer
Nov 1, 1990·The Journal of Infectious Diseases·R B Kimsey, A Spielman
May 11, 1989·Nucleic Acids Research·G S GassmannR Wallich
Dec 1, 1986·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·K Hovind-HougenA Hovmark
Mar 20, 1995·Molecular & General Genetics : MGG·D K AndersonA Newton
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·S C Schuster, S Khan
Aug 1, 1994·Microbiology·I Saint GironsB E Davidson
Aug 11, 1994·Nucleic Acids Research·O Domínguez, C López-Larrea
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S MaloV M Ingram
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S F GoldsteinJ A Kreiling
Oct 29, 1993·Gene·S J RomanP Matsumura
Citations
Aug 29, 2009·Applied Microbiology and Biotechnology·Yuki Tonooka, Masahiro Fujishima
Feb 3, 2005·Nature Reviews. Microbiology·Patricia A RosaPhilip E Stewart
Jan 3, 2001·Molecular Microbiology·E Y DobrikovaF C Cabello
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xiaowei ZhaoJun Liu
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·M A MotalebN W Charon
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·Y ShenP Ronald
May 16, 2002·Infection and Immunity·Jackie McCluskeyDavid G E Smith
May 12, 2009·Journal of Bacteriology·Tanya SouleValerie Stout
Mar 29, 2011·Journal of Bacteriology·M A MotalebJun Liu
May 1, 2012·Journal of Bacteriology·Brandon L JutrasBrian Stevenson
Jun 13, 2000·Journal of Bacteriology·Y K Kim, L L McCarter
Apr 12, 2002·Journal of Bacteriology·Jonathan McClainCarl E Bauer
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L L McCarter
Aug 19, 2011·The FEBS Journal·Claudia LeoniLuigi R Ceci
Jan 26, 2008·FEMS Microbiology Letters·Jinhong WangDavid J Ellar
Jul 16, 2016·Molecular Microbiology·Ki Hwan MoonMd A Motaleb
Nov 22, 2016·Nature Microbiology·Kai ZhangChunhao Li
Oct 12, 2018·Molecular Microbiology·Ki Hwan MoonMd A Motaleb
Aug 15, 1997·FEMS Microbiology Letters·Y Ge, N W Charon
Oct 6, 1999·Molecular Microbiology·M J Kullen, T R Klaenhammer
May 9, 1998·Journal of Bacteriology·Y GeN W Charon
Oct 22, 2020·Journal, Genetic Engineering & Biotechnology·Seyedeh-Samira AshrafmansouriMarie Seidi